Emilio Carbone Carbone

USERN Advisory Board

Born: 12 January 1948, in Alassio (SV), Italy

Family: Married, one son

Home Address: Torino (Italy)

Nationality: Italian

Position: Former Full Professor, now Adjunct Professor, Department of Drug Science, University of Turin, Italy

Lab Address: Lab of Cellular Physiology & Molecular Neuroscience, Corso Raffaello 30,10125, Torino

Email: [email protected]

Links:
http://www.dstf.unito.it/do/gruppi.pl/Show?_id=q8tb
https://www.researchgate.net/profile/Emilio_Carbone
https://orcid.org/0000-0003-2239-6280

Education and training

1971            Degree on Physics with full marks at Genoa University (Italy) with a master thesis with Drs. Franco Conti ed Enzo Wanke on the “1/f noise” properties of solid-state devices for applications to studies on ion current fluctuations in excitable membranes.
1971-73        Two years N.I.H. fellowship at the National Institute of Mental Health in the Laboratory of Neurobiology of Dr. Ichiji Tasaki in Bethesda (MD, USA) to study the molecular properties of squid giant axon excitability using classical electrophysiological recordings and newly developing optical techniques using voltage-dependent fluorescent dyes and aequorin bioluminescence.
1973-74        Research collaborator at the Department of Physics, University of Genoa, Italy, with a research contract for studying under the supervision of Dr. Franco Conti the signaling properties of voltage-dependent dye molecules on artificial bilayer membranes and stimulated squid giant axons.
1974-75        Compulsive military service.
1975            Six months contract at the Institute of Cybernetics and Biophysics, National Research Council in Camogli (Genoa, Italy) to collaborate with Dr. Enzo Wanke on a project on the gating properties of neuronal voltage-gated Na⁺ and K⁺ channels and their modulation by intracellular and extracellular pH changes.
1976            Researcher at the Institute of Cybernetics and Biophysics, National Research Council in Camogli (Genoa, Italy).

Employment and research experience

1976-83          Associate researcher at the Institute of Cybernetics and Biophysics, C.N.R., Camogli, Italy.
1983-85          Associate researcher at the Max-Planck-Institut für Psychiatrie, Abteilung Neurophysiologie, München, Germany.
1986-18          Full professor of Physiology at the Department of Drug Science, University of Torino, Italy.
1986-93          Visiting Professor at the M.P.I. für Psychiatrie, Abteilung Neurophysiologie, München, Germany.
1994              Visiting Professor at the Pacific Biomedical Research Center, Békesy Laboratory of Neurobiology, University of Hawaii, Honolulu, HW, USA.
1994-01          Visiting Professor at the Department of Pharmacology & Terapeutics, Universidad Autonoma de Madrid, Madrid, Spain.
2000-18          Member of the Doctorate School of Neuroscience, University of Torino, Italy.
2001-06          Director of the Research Unit of the National Institute of Physics Matter of the University of Torino.
2005-18          Professor of Physiology at the School of Hospital Pharmacy, Faculty of Pharmacy, University of Torino.
2006-21          Member elected of the Academia Europaea. Section of “Physiology & Medicine”.
2009              Visiting Professor at the Department of Biophysics, Institute of Cellular Physiology, UNAM, México City, México.
2010-11          Member of the Editorial Board of the Pflügers Archiv European Journal of Physiology, Springer Verlag, Berlin
2009-12          Director of the Physiology Section at the Department of Neuroscience, Torino
2011-21          Executive Editor of the Pflügers Archiv European Journal of Physiology, Springer Verlag, Berlin
2014-18          Vice-Director of the Nanostructured Surfaces and Interfaces (NIS) Center of Excellence, University of Torino
2015-18          Vice-Director of Research at the Department of Drug Science, University of Torino, Italy
2018-21          President of the Nanostructured Surfaces and Interfaces (NIS) Center of Excellence, University of Torino
2018-21          Contract professor of Physiology at the Department of Drug Science, University of Torino, Italy.
2019              Guest Professor at the Leopold‐Franzens‐University of Innsbruck to promote top‐level international scientific and didactic exchange with the Center of Chemistry and Biomedicine of the University of Innsbruck and at supporting internationalization on site. (May-June 2019).
2019-21          External advisor of the CavX - Calcium channels in excitable cells PhD Neuroscience program with active groups operating in Innsbruck and Krems (Austria).

Professional experience in the last 15 years

2003           Research Collaboration with the Universidad de la Laguna de Madrid, Department of Physical Medicine & Pharmacology, Tenerife (Spain) for a project on “Amperometric measurements of quantal release of catecholamines in chromaffin cells”
2004           Responsible of the research line: “Cell-adhesion on nanostructured surfaces” at the Centre of Excellence N.I.S., Turin.
2006           Italian partner of the Marie Curie Research Training Network “CavNET”

2006 Local coordinator of a INTAS research project in cooperation with the University of Kiev (Ukraine), and Manchester (UK).

2007-18 Research collaboration with the University of Ulm (Germany), Dept of Electronic Devices and Circuits for a project on neuro-biochips based on diamond-doped surfaces

2007-10 Project Steering Committee member of the European CavNET RT Network

2008-10 Italian partner of a Vigoni project between Italian and German Universities

2009-15 Research Collaboration with the University of Innsbruck (Austria), Dept of Pharmacology & Therapeutics for a project on L-type calcium channels as pacemakers in central neurons and chromaffin cells.

2010-14 Research Collaboration with the Dept. Molecular Physiology of Hearing, University of Tübingen (Germany), for a project on L-type and BK channels co-localization in chromaffin cells and hippocampal neurons.

2011-18 Research Collaboration with the Institute of Functional Genomics (CNRS UMR 5203) and the University of Montpellier (France) for a project on the role of L-type calcium channels in pacemaking central neurons and chromaffin cells.

2011-13 PI of the FESR-POR project “MicroDiBi” funded by the Regione Piemonte with EU funds for the design of new diamond microchips for recording action potential activity and amperometric signaling from neuronal networks and neuroendocrine tissues.

2012-15 PI of the Torino-University project “Novel diamond-based MEA microchips for recordings action potential and amperometric signals from neuronal networks” funded by Intesa-SanPaolo.

2012-14 Co-responsible of the FESR-POR project “Be-Free” funded with EU funds for the design of an assay to detect illegal usage of beta-agonists on bovine cardiac pacemaker cells and in the urine of athletes.

2013-15 PRIN-MIUR (local coordinator) project on: "Excitatory/inhibitory balance in the central nervous system: synaptic transmission, plasticity and synaptopathies".

2015-19 PI of the Telethon grant n. GGP15110: “Bridging Timothy syndrome Cav1.2 calcium channel mutations (TS1 and TS2) to autism spectrum disorders”.

2015-21 Research Collaboration with the University of Innsbruck (Austria), Dept of Pharmacology & Therapeutics for a project on “Role of Cav1.3 channels in the regulation of AP firing in neurosecretory cells and hippocampal neurons”.

2017-21 Research Collaboration with the Universidad Autonoma de Madrid, Department of Pharmacology and Therapeutics, Madrid, Spain on a project on “Muscarinic modulation of ion currents and action potential firing in mouse chromaffin cell”.

Technical, managing and social competence

Technical competence

E. Carbone is group leader and deputy director for research at the Department of Drug Science of Torino. He is presently coordinator of the CNISM Research Unit and is Deputy Director of the Center of Excellence N.I.S. (Nanostructured Interfaces and Surfaces) and to the Scientific Council of the Neuroscience Institute (N.I.T.) in Torino. He has consolidated experience in the physiology and biophysics of neuronal excitability, voltage-gated Ca²⁺ channels, Ca²⁺ channel-secretion coupling and central neurons synaptic activity.

EC has specific interest on: i) Ca²⁺ channel permeability and gating, ii) expression, distribution and role of voltage-gated Ca²⁺ channels at the somatic and presynaptic terminals, iii) role of neurotrophic factors on synaptic plasticity through the recruitment of voltage-gated Ca²⁺ channels, iv) role of Ca²⁺ channels in the regulation of Ca²⁺-secretion coupling in neuroendocrine cells and hippocampal neurons.

Most of the studies are pursued by means of the patch-clamp technique in all its configurations (whole-cell, cell-attached, perforated-patch, membrane capacitance) combined with amperometric recordings. In the specific field of Ca²⁺ channel function and neuronal excitability, EC has made important contributions concerning:

the gating properties of low-threshold T-type Ca²⁺ channels,

the molecular basis of single Ca²⁺ channel inhibition by membrane receptors and G proteins,

the direct and remote modulation of neuroendocrine L-type channels,

the effects of Ca²⁺ channel modulation, up-regulation and recruitment during stress conditions on neurotransmitter release and cell exocytosis,

the role of L-type channels as pacemaker channels in chromaffin cells and central neurons,

the effects of L-type channels inactivation on neuronal development, cell excitability and synaptic plasticity

In collaboration with the Physics Department in Torino and with the Institute of Electron Devices and Circuits of Ulm University, Ulm (Germany), EC has contributed in the last ten years (2010-20) to the development of new diamond-based biosensors able to detect amperometric, potentiometric and optical signals to be used for the simultaneous detection of quantal release of neurotransmitter molecules (adrenaline, noradrenaline, dopamine and serotonin), action potential waveforms and fluorescence calcium signals from neuroendocrine cells and neurons. Sixteen full papers have been published on this argument (see Publications on the last 10 years)
EC is author of 172 full papers on international journals and books with h-index 51, h10-index 130 and 8727 quotations (taken from Google Scholar). EC is invited speaker in national and international congresses, workshops, PhD courses, advanced schools and seminars.

A list of recent invited lectures is given:

2005 International Workshop on:Ca²⁺ channels in health and disease, Tübingen (Germany)

2005 Biennial meeting of the MIUR-PRIN projects. GlaxoSmithKline Center, Verona (Italy)

2005 Physiological Society International Workshop on: Ion channel: from Physiology to Pathology, Sevilla (Spain)

2005 1st International Neuroscience Meeting, Timisoara (Romania)

2005 MMD Meeting, workshop on: Biophysics of excitable cells, Genova (Italy)

2006 13th International Symposium on: Chromaffin Cell Biology, Puchón (Chile)

2006 Symposium on: “25 years of giga-seal patch clamping”, Heidelberg (Germany)

2006 Fronteras en Terapeutica IV. Course on: Comunicacion neuronal, Molina de Segura, Murcia (Spain)

2006 International workshop on:L-type Calcium channels in health and disease, Tübingen (Germany)

2007 NIS Colloquium on: “Carbon nanotubes and carbon hybrid/composites”, NIS Centre of Excellence, Torino (Italy)

2007 Segnali di Ca²⁺ in Piemonte, DISCAF, Novara (Italy)

2007 1^st International Conference on: Calcium channels, Moorea Pearl Resort, Tahiti (France Polinesia)

2007 Martin Morad’s 70^th Anniversary Symposium on: Calcium signaling, Mount Desert Island, Main (USA)

2007 International Symposium on: Calcium Channels and Transporters, Conference Centre, Manchester (UK)

2007 14^th International Symposium on Chromaffin Cells Biology, Sestri Levante (Italy)

2007 Invited lecture on: Ca²⁺ channels and exocytosis in neurosecretory cells. IZN, Heidelberg University, Heidelberg (Germany)

2007 Invited lecture on: T-type and L-type channels in chromaffin cells: role in pace-making and secretion. Technische Universitad, München (Germany)

2007 1^st Annual CavNET meeting on: Ion channels, Montpellier (France)

2008 Conferenza su: Segnali di calcio, sinapsi neuronali nanotecnologie: nuovi approcci e future convergenze. Istituto Nazionale di Ricerca Metrologica (I.N.R.I.M.), Torino (Italy)

2008 Advanced Workshop T-type calcium channels: from discovery to channelopathies, 25 years of research. Kyiv (Ukraine)

2008 2^nd Annual CavNET meeting on: Ion channels, University Center Obergurgl, Obergurgl (Austria)

2008 Invited lecture on: Ca²⁺ signaling by L-type channels in chromaffin cells. Instituto de Fisiologìa Celular, UNAM, Ciudad de México (México)

2008 Invited lecture on: Calcium channels at work in chromaffin cells: modulation and recruitment during stress-mimicking stimuli. LI Congreso Nacional de Ciencias Fisiológicas, Merìda, Yucatan (México)

2009 Le meraviglie dei nanomateriali, Circolo dei Lettori, Torino (Italy)

2009 Workshop on: Ca_v1.3 Selective Ca²⁺ channel blockers, Innsbruck, (Austria)

2009 International Conference on: Molecular mechanisms of intracellular calcium signalling, Kiev (Ukraine)

2009 15^th International Symposium on Chromaffin Cells Biology, Mérida, Yucatan (México)

2009 3^rd Annual CavNET meeting on: Ion channels, Sestri Levante (Italy)

2010 2^nd International Conference on: Calcium channels, The Inn at Robert’s Grove, Placencia (Belize)

2010 Settimana del Cervello 2010, Circolo dei Lettori, Torino (Italy)

2010 4^th Annual CavNET meeting on: Ion channels. UCL, London (UK)

2010 Invited lecture on: Canales de calcio L y T: marcapasos de la neurona. IX Escuela de Farmacologia “Teofilo Hernando”, Santander (Spain)

2011 1^st CavNET follow-up meeting on: L-type channels in Health & Disease. Hearing Research Center Tübingen, Tübingen (Germany)

2011 16^th International Symposium on Chromaffin Cell Biology, Beijing (China)

2011 62^o Congresso Società Italiana di Fisiologia, Sorrento (Italy)

2012 1^st European Calcium Channel Conference, Alpbach (Austria)

2012 2^nd CavNET follow-up meeting on: L-type channels in Health & Disease. Department of Drug Science, Torino (Italy)

2012 Workshop on Biosensors & correlated devices: XCVIII Congress of the Italian Physics Society, Napoli (Italy)

2013 3^rd International Conference on: Calcium channels, Koh Lanta (Southern Thailand)

2013 Invited lecture on: L-type calcium channels as controllers of pace-making, secretion and endocytosis in chromaffin cells. Medical University of Vienna, Center of Physiology and Pharmacology, Wien (Austria)

2013 Invited lecture on: Recording of pacemaker currents and secretion in neurons and chromaffin cells, Innsbruck Univ, Center for Chemistry & Biomedicine, Innsbruck (Austria)

2013 17^th International Symposium on Chromaffin Cell Biology, Rouen, (Normandy, France)

2013 3^rd CavNET follow-up meeting on: L-type channels in Health & Disease. Slovak Cultural Institute, Jilská 450/16, Prague (Slovakia)

2014 Symposium on: Dagli atomi al Cervello: Le Scienze di Base per la comprensione delle funzioni del cervello. Politecnico di Milano, Milano (Italy)

2014 Special Guest Lecturer at the VI Congress of Ukrainian Society for Neuroscience. June 2014, Kiev Ukraine

2014 4^th CavNET follow-up meeting on: L-type channels in Health & Disease. Technische Universität Kaiserslautern Kaiserslautern (Germany)

2014 Presentation on: Biosensing neuronal signaling: a challenge for diamond-based lab-on-chip. 2^nd Diamond & New Technologies, Dept of Physics, University of Torino (Torino)

2015 2^nd European Calcium Channel Conference, Chairman and speaker on: Cav1.3 drives pacemaking and firing-modes in adrenal chromaffin cells.). May 2015, Alpbach (Austria)

2015 International Symposium on Ion Channels Trimming the Brain: Cav1.3, BK and SK channels as regulators of neuron-like firing in neurosecretory cells. September 2015, Kyiv (Ukraina)

2015 International Congress of FisMat2015. Chairman of the section on: Diamond-based substrates and Memristors. September 2015, Palermo (Italy)

2015 10th SICC International Tutorial Workshop on "Topics in Nonlinear Dynamics”. Monitoring neuronal complexity and channelopathies using classical and newly designed neurobiosensors. Torino Polytechnic, September 2015. Torino (Italy)

2016 Simposio-homenaje al Prof. Antonio García García, Facultad de Medicina de la Universidad Autónoma de Madrid, 15 Sept. 2016

2016 4th International Congress on Voltage-gated Calcium Channels. Hoi An (Vietnam), 2-7 Oct 2016

2017 Invited lecture on: “Neuronal firing: role of Cav, Nav, Kv and KCa channels” at the Center of Chemistry and Biomedicine University of Innsbruck, Innsbruck (Austria)

2017 Invited lecture on: Cell signaling and secretion in adrenal chromaffin cells: specific roles for Na+, K+and Ca2+channel gating” at the MolTag Seminar, Dept. of Pharmacology and Toxicology, University of Vienna, Wien (Austria)

2017 19^th International Symposium on Chromaffin Cell Biology, Sheffield, (UK)

2018 3^rd European Calcium Channel Conference, Chairman and speaker on: Cav1.2 and Cav1.3 channel gating mutations inducing autism in humans and mouse models. Alpbach (Austria). May 2018.

2018 Invited lecture on: “L-type calcium channels in pacemaking, secretion and autism viewed through mice models” at the Institut de Génomique Fonctionnelle (IGF), CNRS UMR 5203, Inserm U1191 34094 (Montpellier), November 28 2018.

2019 5th International Congress on Voltage-gated Calcium Channels. George Town (Penang, Malaysia), March 31- April 5 2019.

2019 Invited presentation on: “Ca2+-signaling dysfunctions of L-type Cav1 channel missense mutations causing ASD: gating defects and therapeutic action” at the EJP meeting on Rare Diseases Info Day and Brokerage Event. TÜBITAK & Sabancı University, İstanbul. November 7 2019.

2020 Invited lecture on: “The role of calcium channels in adrenal chromaffin cells” at the Symposium on “Ion channels in brain diseases: causes, mediators and drug targets” at Karl Landsteiner University of Health Sciences, Krems an der Donau, February 27 2020.

2021 Invited virtual lecture on: “Cav1.2 channelopathies causing autism: new molecular and cellular hallmarks on Timothy syndrome” for the Neuroscience Seminar Series at University of Massachusetts, Medical School, Worcester (MA, USA), February 11, 2021

EC is active reviewer of papers submitted to biophysical, physiology, pharmacology and neuroscience international journals, including: The Biophysical Journal, Biosensors, European Biophysics Journal, Biosensors & Bioelectronics, Scientific Reports, Nature Communications, Cell Calcium, Journal of Physiology, Journal of General Physiology; European Journal of Physiology, Physiological Reviews, Journal of Cellular Physiology, American Journal of Physiology, Journal of Biological Chemistry, Brain Research, Journal of Neurophysiology, Journal of Neuroscience, European Journal of Neuroscience, Neuroscience, Journal of Neuroscience Methods, Journal of Neurochemistry, Journal of Neuropharmacology, Molecular Pharmacology, Journal of Pharmacology and Experimental Therapeutics, FASEB Journal, Journal of Clinical Investigation, Plos One, Cell Death & Diseases, and Biochimica Biophysica Acta Biomembranes,

EC is elected Executive Editor of Pflügers Archiv European Journal of Physiology.

EC has reviewed also grant applications for national and international organisations (Human Frontier (UE), Wellcome Trust (UK), FECYT (Spain), National Science Foundation (USA), Slovak Research and Development Agency, Israel Science Foundation (Israele), BBSRC funds (UK), Creative Research Initiative Program (North Korea), AFM-Téléthon (France), Slovakian Agency of Research (Slovakia), FWF der Wissenschaftsfonds (Austria), Agence Nationale de la Recherche (France), Italian Telethon (Italy), Ministero dell’Istruzione, Università e Ricerca (Italy), Ministero delle Attività Produttive (Italy). Recently he reviewed also ERC consolidator, Europ. Science Found. (ESF) and Czech Science Found. (GAČR) grants.

Orgazining and social competence

In the period 2001-2005 EC is Director of the Research Unit at the “Istituto Nazionale di Fisica della Materia” (I.N.F.M.) of Torino University. In 2002 he has been appointed member of the Evaluation Committee of the Ramon y Cajal Programme organised by the Spanish Foundation for Science and Technology (FECYT) for recruiting new investigators of quality in the area of Physiology and Pharmacology. In 2003 he was consultant of the Ministry of Industry to evaluate two projects concerning two industrial research projects of the II Bando PIA INNOVAZIONE. Since 2004 he is member of the Scientific Council of Centre of Excellence N.I.S. (Torino) and representative of the University Rector of Torino at the C.N.I.S.M (Rome). In 2004 he was also member of the CIVR Committee of the University of Torino for the area of Nanotechnology. Since 2005 he is member of the Scientific Council of the Neuroscience Institute of Turin. Since 2015, EC is member of the ANVUR (National Agency for the Evaluation of National Universities and Research Institutes; committee section on Health Care) for the 3^rd mission of Italian Universities.

In the past, EC has been the local coordinator of two projects funded by the CNR and two project FIRB funded by MIUR in the field of Neuroscience and Nanobiotechnologies. He has been for a decade local coordinator in Torino of national PRIN-MIUR projects focused on the biophysics and physiology of ion channels. Since 2005 is national coordinator of the PRIN-MIUR project: "Neuronal sodium, calcium and potassium channels: physiological role and channelopathies". He is also principal investigator of the 2004-CIPE project funded by Regione Piemonte: “Biosensors and interaction between neurons and nanostructured surfaces”. Recently (2006) he became partner of the European Marie-Curie Research Training Network “CavNET” that joins university laboratories from all Europe interested on the function and pathological diseases associated to L-type calcium channels. He is also western member of an INTAS project in collaboration with research groups of Kiev University and Manchester Biological School. He was responsable of the POR-FESR project MicroDiBi funded by the Regione Piemonte for the design of new diamond microchips for recording action potential waveforms, amperometric and optical signals from neuronal networks. EC is now running a Telethon project for studying the molecular basis of the Timothy Syndrome, a rare disease associated to the mutations of Cav1.2 calcium channels.

EC has organized and is organizing several local and international meetings:

2001          A two days workshop on: Structure and function of membrane channels. INFM UdR-University, Torino (Italy)
2002          Workshop of the INFM Research Unit Torino University, Physics Department, Torino (Italy)
2005          Joint Symposium SIF - Physiological Society on: Calcium signaling: from Physiology to Pathology, Palermo (Italy)
2005          NIS Colloquia on: “Cells and biomolecules on nanostructured surfaces”. Torino (Italy)
2007          Segnali di Calcio in Piemonte, Novara (Italy)
2007          14^th International Symposium on Chromaffin Cells Biology, Fondazione Mediaterraneo, Sestri Levante (Italy)
2009          Symposium on: Ion channels and secretion in chromaffin cells.15^th International Symposium on Chromaffin Cells Biology, Mérida, Yucatan (México)
2009          Summer School and 3^rd Annual Meeting of Cavnet, Fondazione Mediaterraneo, Sestri Levante (Italy)
2011          Symposium on: Voltage-gated calcium channels and cell function. 16^th International Symposium on Chromaffin Cells Biology, Bejing (China)
2011          Symposium Voltage-gated L-type (Cav1) calcium channels in health and diseases at the 62^o Congresso Società Italiana di Fisiologia, Sorrento (Italy)
2012          NIS Colloquia on: Structure and function of Cav1.2 and Cav1.3 L-type channels. Torino-Esposizioni, Torino (Italy)
2013          Symposium on: Calcium and ion channels. 17^th International Symposium on Chromaffin Cells Biology, Rouen (France)
2013          Symposium on: New and classical biosensors for recording cellular and neuronal signaling, FisMat13, Milano (Italy)

Qualifications to collaborate with foreign laboratories and industrial partners

The Lab directed by EC is fully equipped for running experiments on the electrophysiology, biophysics and pharmacology of central and peripheral neurons, chromaffin cells, -pancreatic cells, clonal cell lines and stem cells that could be interesting for industrial partners such as pharmaceutical companies or industries who are interested in the health hazard of nanostructured and ultrafine particles on neurons and excitable biological tissues. In general, the Lab can undertake any kind of collaborative research activity with neuroscience groups and any type of industries that are interested to the acute and chronic effects of most organic and inorganic chemical substances, physical treatment or biological assay on the functioning and survival of excitable cells.

In the past EC has been consultant at the Bayern AEG (Wuppertal, Germany) and is presently collaborating with the Eli Lilly Company (Surrey, UK) on a project based on the effects of drugs on Ca²⁺ channel gating. The Lab has received grants from industries for carrying out preliminary screening tests of various Ca²⁺-antagonists for developing antiarrhythmic and antihypertensive drugs. The Lab has also been funded for several years with grants from Telethon for studies on the role of voltage-gated Ca²⁺ channels in motoneurones degeneration as a possible cause of Amyotrophic Lateral Sclerosis.

The lab-team presently includes post-doc fellows, PhD students, graduate students and technicians who are trained to prepare cell cultures, undertake complex electrophysiological recordings (intracellular and extracellular action potentials, membrane currents, synaptic signals, fluorescence intracellular calcium measurements) and analyze most of the data with up-to-date computer programs. The Lab includes: 1 associate professor, 1 researcher, 2 post-docs, 1 PhD student, 4 undergraduate students and 1 technician. PhD and post-docs are supported by the MIUR (FIRB giovani) and Telethon Foundation to work on scientific projects related the physiology and rare diseases of voltage-gated Ca²⁺ channels and development of new diamond-based microchips for recording neuronal excitability.

Research funding in the last 20 years

2001-03 PRIN-MIUR (local coordinator) "Calcium channels channelopathies"

2001-03 Marie Curie post-doc position for a project on the "Modulation of single Ca²⁺ channels in chromaffin cells”

2001-03 INTAS Project "Intracellular Calcium signaling"

2001-03 Cavalieri Ottolenghi Foundation grant for a project on the "Properties of inibitory hippocampal neurons"

2001-03 CNR strategic project on “Neuroscience”

2002-05 FIRB MIUR (local coordinator) project on "Nanobiotechnologies"

2003-05 PRIN-MIUR (local coordinator) "Calcium channels channelopathies"

2003-05 FIRB MIUR project on "Neuroscience"

2001-05 Grants from the University of Torino (ex 60%). Projects on "Calcium channel functioning and modulation”

2001-05 INFM grant on the "Biophysics of voltage-gated Ca²⁺ channels"

2004-05 MIUR funds to the NIS Center of Excellence for a project on "Cellular adhesion and biosensors"

2004-06 SanPaolo Imi Foundation funds to the NIS for a project on “Neuronal extracellular recordings using multi-electrode arrays"

2005-07 PRIN-MIUR (national coordinator), project on: "Neuronal sodium, calcium and potassium channels: physiological role and channelopathies"

2005-08 CIPE-Regione Piemonte (principal investigator), project on “Biosensors and interaction between neurons and nanostructured surfaces”

2006-10 1^st European grant for a Marie-Curie Research Training Network “CavNET” (1 PhD, 1 post-doc position) on “L-type channels in health and diseases”

2007-09 INTAS project between the University of Manchester-Kiev and Torino

2007 IBRO, Int. Neurochemistry Society, Compagnia di SanPaolo grants for the organization of the 14^th International Symposium on Chromaffin Cells Biology, Sestri Levante, Italy

2007-10 CIPE-Regione Piemonte (co-presenter), project “Nanoparticles: from their impact on the environment and human health to safer production and usage” (acronym: NANOSAFE)

2007-10 2^nd European grant for a Marie-Curie Research Training Network “CavNET” (1 PhD, 1 post-doc position) on “L-type channels in health and diseases”

2007-10 Compagnia di SanPaolo: grant to the NIS Center of Excellence for a project on “Neurons-on-chips and biosensors"

2007-09 IMAGEEN project between Torino and Teotuan (Morocco) University on the: “Action of cannabinoids on calcium channels and neurosecretion in central neurons and chromaffin cells”

2007-09 PRIN-MIUR (national coordinator) "Neuronal sodium, calcium and potassium channels: physiological role and channelopathies"

2007-09 Project Alfieri on the nanotechnologies “Nanoparticles and potential toxicity at the nervous system: an approach integrated to the definition of relevant parameters”

2008-09 Cavalieri-Ottolenghi Foundation: grant for the acquisition of a fluorescence microscope for calcium imaging on neuronal networks

2008-10 Vigoni project funded by the Italian-German University: “Planar microelectrodes for amperometric and potentiometric registrations from secretory cells”

2011-13 POR-FESR project MicroDiBi funded by the Regione Piemonte for the design and test of: “Diamond microchips for drug-screening and biomedical applications”

2011-13 Compagnia di SanPaolo grant to the NIS Center of Excellence on for a project on “Advances in nanostructured materials and interfaces for key technologies” “Diamond biosensors for recording neuronal activity"

2012-14 POR-FESR project BE-FREE funded by the Regione Piemonte for the use of: “A rapid and accurate method to detect illegal usage of beta-agonists”

2013-16 PRIN-MIUR (local coordinator) project on: "Excitatory/inhibitory balance in the central nervous system: synaptic transmission, plasticity and synaptopathies".

2015-18 PI of the Telethon grant n. GGP15110 on: “Bridging Timothy syndrome Cav1.2 calcium channel mutations (TS1 and TS2) to autism spectrum disorders”.

2019-20 Member of the local Unit of Torino involved in the grant EU-FET OPEN - GA 828946 on: “Photonic and nanometric high-sensitivity bio-sensing (PATHOS)”

Publications in international journals in the last 10 years (2011-2020)

Marcantoni A, Vandael DH, Mahapatra S, Carabelli V, Sinnegger-Brauns MJ, Striessnig J, Carbone E. 2010. Loss of Cav1.3 channels reveals the critical role of L- and BK-channel coupling in pace-making mouse adrenal chromaffin cells. Journal of Neuroscience 30(2): 491-504

Gao Z, Carabelli V, Carbone E, Colombo E, Demaria F, Dipalo M, Gosso S, Manfredotti C, Pasquarelli A, Rossi S, Xu Y, Vittone E, Kohn^.E. 2010. Transparent diamond microelectrodes for biochemical application. Diamond & Related Materials 19: 1021–1026

Carabelli V, Gosso S, Marcantoni A, PasquarelliA, Xu Y, Colombo E, Gao Z, Scharpf J, Kohn E, Carbone E. 2010. Nanocrystalline diamond microelectrode arrays fabricated on sapphire technology for high-time resolution of quantal catecholamine secretion from chromaffin cells. Biosensors & Bioelectronics 26: 92-98

Comunanza V, Marcantoni A, Vandael DH, Mahapatra S, Gavello D, Carabelli V, Navarro-Tableros V, Carbone E 2010. Cav1.3 as pacemaker channels in adrenal chromaffin cells: specific role on exo- and endocytosis? Channels (Austin)4: 440-446

Vandael D, Marcantoni A, Mahapatra S, Caro A, Ruth P, Zuccotti A, Knipper M, Carbone E. 2010. Cav1.3 and BK channel coupling for timing and regulating cell firing. Molecular Neurobiology 42: 185-198

Gao Z, Carabelli V, Carbone E, Colombo E, Manfredotti C, Pasquarelli A, Feneberg M, Thonke K, Vittone E, Kohn E. 2011. Transparent microelectrode array in diamond technology. Journal of Micro-Nano Mechatronics 6: 33-37

PasquarelliA, Carabelli V, Xu Y, Colombo E, Gao Z, Scharpf J, Carbone E, Kohn E. 2011. Diamond microelectrodes arrays for the detection of secretory cell activity. International Journal of Environmental Analytical Chemistry 91: 150-160

Mahapatra S, Marcantoni A, Vandael DHF, Striessnig J, Carbone E. 2011. Are Cav1.3 pacemaker channels in chromaffin cells? Possible bias from resting cell conditions and DHP blocker usage. Channels (Austin) 5: 219-224

Colombo E, Men Y, Scharpf J, Pietzka C, Dipalo M, Herfurth P, Gao Z, Schneider M, Carabelli V, Carbone E, Kohn E, Pasquarelli A.^.2011. NCD Microelectrode array with sub-cellular spatial resolution. Diamond & Related Materials 20: 793-797

Scott RS, Bustillo-Merino D, Olivos-Oré LA, Cuchillo-Ibañez I, Barahona MV, Carbone E, Artalejo AR. 2011. Contribution of BK channels to action potential repolarisation at minimal cytosolic Ca²⁺ concentration in chromaffin cells. Pflügers Archiv European Journal of Physiology 462: 545-557

Gosso S, Gavello D, Natale G, Giachello C, Franchino C, Carbone E, Carabelli V. 2011. The effect of CdSe-ZnS quantum dots on calcium currents and catecholamine secretion in mouse chromaffin cells. Biomaterials 32: 9040-9050

ComunanzaV, Carbone E, Marcantoni A, Sher E, Ursu D. 2011. Calcium-dependent inhibition of T-type calcium channels by TRPV1 activation in rat sensory neurons (submitted to Pflügers Archiv European Journal of Physiology 462: 709-722

GavelloD, Vandael DH, Cesa R, Premoselli F, Marcantoni A, Cesano F, Scarano D, Fubini B, Carbone E, Fenoglio I, Carabelli V. 2012. Altered excitability of cultured chromaffin cells following exposure to multi-walled carbon nanotubes. Nanotoxicology 6: 47–60

Mahapatra S, Calorio C, Vandael DHF, Marcantoni A, Carabelli V, Carbone E. 2012. Calcium channel types contributing to chromaffin cell excitability, exocytosis and endocytosis. Cell Calcium 51: 321– 330

Gavello D, Rojo-Ruiz J, Marcantoni A, Carbone E, Carabelli V. 2012. Leptin counteracts the hypoxia-induced inhibition of spontaneously firing hippocampal neurons: a microelectrode array study. Plos One 7(7): e41530

Guérineau NC, Desarménien MG, Carabelli V, Carbone E. 2012. Functional chromaffin cell plasticity in response to stress: focus on nicotinic, gap junction and voltage-gated Ca²⁺ channels. Journal of Molecular Neuroscience 48(2): 368-386

Carbone E, Guérineau NC, Peng Loh Y, Zhou Z. 2012. Commentary: Ion channels, fusion pores and exocytosis. Journal of Molecular Neuroscience 48(2): 357-359

Mahapatra S, Marcantoni A, Zuccotti A, Carabelli V, Carbone E. 2012. Equal sensitivity of Cav1.2 and Cav1.3 channels to the opposing modulations of PKA and PKG in mouse chromaffin cells. The Journal of Physiology 590.20: 5053-5073

Vandael DHF, Marc

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