Juan Saéz
USERN Advisory Board
DEGREES:
Ph.D. in Neuroscience, Albert Einstein Coll. Med., Yeshiva Univ., Bronx, N.Y. (1983-1986).
Biochemist (equivalent to MS in Biochemistry), Univ. de Concepción, Concepción. Chile (1974-1979).
ACADEMIC POSITIONS:
2018-present. Professor, Neurosci. Institute, Univ. de Valparaíso, Chile.
2004-2021. Professor, Dept. Physiology, Pontificia Univ. Católica de Chile (PUCC), Santiago, Chile.
2007-present. Visiting Professor, Dept. Neuroscience, Albert Einstein Coll. Medicine (AECOM), Yeshiva Univ., Bronx, N.Y., USA.
1994-Nov. 2004: Associate Professor, Dept. Physiology, PUCC, Santiago, Chile.
1994-2007: Visiting Associate Professor, Dept. Neuroscience, AECOM, Yeshiva Univ., Bronx, N.Y., USA.
1993-1994: Assistant Professor, Dept. Physiology, PUCC, Santiago, Chile.
1989-1993: Assistant Professor, Dept. Neuroscience, AECOM, Yeshiva Univ., Bronx, N.Y., USA.
1987-1989: Instructor, Dept. Neuroscience, AECOM, Yeshiva Univ., Bronx, N.Y., USA.
1986-1987: Post-doctoral fellow, Dept. Neuroscience, AECOM, Yeshiva Univ., Bronx, N.Y., USA.
1979 -1983: Instructor, Dept. Physiology, Univ. de Concepción, Chile.
ADMINISTRATIVE POSITIONS:
2017-presente. Deputy Director of the Neuroscience Interdisciplinary Center of Valparaíso.
2019-present. Member of the Human Capital Training Advisory Committee ANID.
2015-2021. Coordinator of Magister, Doctorate and Post-doctorate scholarships in Physiology and iochemistry. CONICYT.
2018-2019. Honorary Professor in the School of Public Health of the University of Hong Kong.
2017-present. Deputy-Director of the Neuroscience Interdisciplinary Institute of Valparaíso.
2015-2021. Coordinator of Ph.D. and Post-doctoral fellowships in Physiology and Biochemistry. CONICYT.
2013-2019. Committee member of the Ph.D. program in Neuroscience. Pontificia Univ. Católica de Chile, Santiago, Chile.
2003-2014. Director of the Ph.D. Program in Physiological Sciences. Pontificia Univ. Católica de Chile, Santiago, Chile.
2009-2019. Subdirector of the Center for Neuroscience, Pontificia Universidad Católica de Chile.
AWARDS AND DISTINCTIONS:
2019. International Member of the National Academy of Science (NAS) USA.
MEMBERSHIP OF SCIENTIFIC SOCIETIES:
1985-present Latin American Biophysical Society.
PUBLICATIONS (ISI) (Google scholar Total citations =16.759, H= 65)
188. Stretch-induced activation of pannexin 1 channels can be prevented by PKA-dependent phosphorylation
BOOKS AND SPECIAL EDITIONS:
BOOK CHAPTERS AND OTHER PUBLICATIONS (NON-ISI).
44. In: BOOK TITLE & ISBN: "Biology of Trypanosoma cruzi," 978-1-83968-204-9. Book Chapter: Possible Role
Ph.D. in Neuroscience, Albert Einstein Coll. Med., Yeshiva Univ., Bronx, N.Y. (1983-1986).
Biochemist (equivalent to MS in Biochemistry), Univ. de Concepción, Concepción. Chile (1974-1979).
ACADEMIC POSITIONS:
2018-present. Professor, Neurosci. Institute, Univ. de Valparaíso, Chile.
2004-2021. Professor, Dept. Physiology, Pontificia Univ. Católica de Chile (PUCC), Santiago, Chile.
2007-present. Visiting Professor, Dept. Neuroscience, Albert Einstein Coll. Medicine (AECOM), Yeshiva Univ., Bronx, N.Y., USA.
1994-Nov. 2004: Associate Professor, Dept. Physiology, PUCC, Santiago, Chile.
1994-2007: Visiting Associate Professor, Dept. Neuroscience, AECOM, Yeshiva Univ., Bronx, N.Y., USA.
1993-1994: Assistant Professor, Dept. Physiology, PUCC, Santiago, Chile.
1989-1993: Assistant Professor, Dept. Neuroscience, AECOM, Yeshiva Univ., Bronx, N.Y., USA.
1987-1989: Instructor, Dept. Neuroscience, AECOM, Yeshiva Univ., Bronx, N.Y., USA.
1986-1987: Post-doctoral fellow, Dept. Neuroscience, AECOM, Yeshiva Univ., Bronx, N.Y., USA.
1979 -1983: Instructor, Dept. Physiology, Univ. de Concepción, Chile.
ADMINISTRATIVE POSITIONS:
2017-presente. Deputy Director of the Neuroscience Interdisciplinary Center of Valparaíso.
2019-present. Member of the Human Capital Training Advisory Committee ANID.
2015-2021. Coordinator of Magister, Doctorate and Post-doctorate scholarships in Physiology and iochemistry. CONICYT.
2018-2019. Honorary Professor in the School of Public Health of the University of Hong Kong.
2017-present. Deputy-Director of the Neuroscience Interdisciplinary Institute of Valparaíso.
2015-2021. Coordinator of Ph.D. and Post-doctoral fellowships in Physiology and Biochemistry. CONICYT.
2013-2019. Committee member of the Ph.D. program in Neuroscience. Pontificia Univ. Católica de Chile, Santiago, Chile.
2003-2014. Director of the Ph.D. Program in Physiological Sciences. Pontificia Univ. Católica de Chile, Santiago, Chile.
2009-2019. Subdirector of the Center for Neuroscience, Pontificia Universidad Católica de Chile.
AWARDS AND DISTINCTIONS:
2019. International Member of the National Academy of Science (NAS) USA.
2017. Academic Member of the Latin American Academy of Science (ACAL).
2017. Outstanding work in the field of transfer of research results, Directorate of Transfer and Development of the Vice-rectory for Research.
2014. Winner of the VII UC Patent Contest. "Identification, compositions and therapeutic uses of heterocyclic blockers of hemichannels formed by connexins".
2010, Winner of the II Competition of Intellectual Property, Pontificia Universidad Católica de Chile
2008, Chilean Scopus price given by Elsevier Editorial. The Chilean Scientist with the highest productivity and impact in Pharmacology, Biochemistry, Genetics and Molecular Biology.
1993, Glaxo Research Institute. Fellowship Award.
1987, Grass Fellowship to work as independent Scientist at the Marine Biological Laboratory.
1980, Best Thesis work of the year given by the Society of Pharmacists and Biochemists of Concepción.
MEMBERSHIP OF SCIENTIFIC SOCIETIES:
1985-present Latin American Biophysical Society.
1990-1994 Biophysical Society
1990-2015 American Society for Cell Biology, USA
1994-present. Chilean Society for Physiology.
1994-present. Chilean Society for Biology.
1994-present. Chilean Society for Cell Biology.
2003-present. Chilean Society for Neuroscience.
2003-present. Free Radicals and Antioxidants Group-Chile.
2019-present. Society of General Physiology, USA
PUBLICATIONS (ISI) (Google scholar Total citations =16.759, H= 65)
188. Stretch-induced activation of pannexin 1 channels can be prevented by PKA-dependent phosphorylation
López X, Escamilla R, Fernández P, Duarte Y, González-Nilo F, Palacios-Prado N, Martínez AD, Sáez JC.
Int J Molec Sci 2020 Dec 2;21(23):E9180. doi: 10.3390/ijms21239180.
187. Choi EJ, Palacios-Prado N, Sáez JC, Lee J. Identification of Cx45 as a major component of gap
junctions in HeLa cells. Biomolecules. 2020 Sep 29;10(10):E1389.
186. Recabal A, Fernández P, López S, Ordenes P, Elizondo R, Farkas C, Uribe E, Caprile T, Saéz JC,
García-Robles MA. FGF2-induced tanycyte proliferation involves a connexin 43-hemichannel/purinergic
pathway. J. Neurochem. . 2020 Sep 16. doi: 10.1111/jnc.15188. Online ahead of print.
185. Cea LA, Fernández G, Arias GB, Castillo-Ruiz M, Brañes MC, Sáez JC. Blockade of hemichannels
normalizes the differentiation fate of myoblasts and features of skeletal muscles from dysferlin-deficient
mice. Int J Molec Sci. 2020 Aug 21;21(17):E6025.
184. Balboa E., Saavedra F., Cea L.A., Ramírez V., Escamilla R., Vargas A., Regueira R., Sáez J.C.
Vitamin E blocks connexin hemichannelsand prevents deleterious effects of glucocorticoid treatment on
skeletal muscles. Int. J. Molec. Sci. 2020, Jun 8;21(11):E4094.
183. Sáez J.C., Vargas A.A., Ortiz F.C., D.E., Giaume C., Orellana J.A. Permeation of molecules through
astroglial connexin 43 hemichannels is modulated by cytokines with parameters depending on the permeant
species. Int. J. Molec. Sci. 2020, Jun 1;21(11):E3970.
182. Fernández G., Arias G.B,, Bevilacqua J.A., Castillo M., Caviedes P, Sáez J.C., Cea L.A. Myofibers
deficient in connexins 43 and 45 expression protect mice from skeletal muscle and systemic dysfunction
promoted by a dysferlin mutation. Biochem. Biophys. Acta- Molecular Basis of Disease 1866 (8) 165800.
(2020).
181. Sáez J.C., Contreras-Duarte S., Labra V.C., Santibañez C.A., Mellado L.A., Inostroza C.A., Alvear T.F.,
Retamal M.A., VelardeV., Orellana J.A. Interferon-γ and high glucose-induced opening of Cx43
hemichannels causes endothelial cell dysfunction and damage. Biochem. Biophys. Acta. Molecular Cell
Research 14;1867(8):118720 (2020).
180. Cisterna CB., Vargas A.A., Puebla C., Lagos C.F., Escamilla R., Matus M-F., Vilos C., Cea L.A.,
Barnafi E., Gaete H., Escobar D.F., Cardozo C., Sáez J.C. Active acetylcholine receptors prevent the atrophy
of skeletal muscles and favor reinnervation. Nature Comm. 11: 1073, (2020).
179. Li T, Niu J, Ezan P., Koulakoff A., Yi C., Sáez JC, Giaume C., Xiao L. The deletion of connexin 43 in
astrocytes promotes CNS remyelination by inhibiting local inflammation. Glia 68(6):1201-1212 (2020).
178. Giaume C., Naus C, Sáez JC, Leybaert L. Glial connexins and pannexins in healthy and diseased brain.
Physiol Rev. 2021: 101(1):93-145.
177. Harcha PA., López X, Sáez PJ, Fernández P, Barría I, Sáez JC. Pannexin-1 channels are essential for
mast cell degranulation triggered during Type I hypersensitivity reactions. Frontiers Immunol, 29 November
2019 https://doi.org/10.3389/fimmu.2019.02703.
176. Asencio-Barría C, Defamie N, Sáez JC, Mesnil M, Godoy AS. Direct Intercellular Communications and
Cancer: A Snapshot of the biological roles of connexins in prostate Cancer. Cancers (Basel). 2019 Sep
14;11(9). pii: E1370.
175. Gómez GI, Velarde V, Sáez JC. Role of a RhoA/ROCK-Dependent Pathway on Renal Connexin43
Regulation in the Angiotensin II-Induced Renal Damage. Int J Mol Sci. 2019 Sep 7;20(18).
174. Eugenín EA, Valdebenito S, Gorska AM, Martínez AD, Bitran M, Sáez JC. Gap junctions coordinate
the propagation of glycogenolysis induced by norepinephrine in the pineal gland. J Neurochem. 2019 Aug 5.
doi: 10.1111/jnc.14846. [Epub ahead of print].
173. Cea LA, Balboa E, Vargas AA, Puebla C, Brañes MC, Escamilla R, Regueira T, Sáez JC.
De novo expression of functional connexins 43 and 45 hemichannels increases sarcolemmal permeability of
skeletal myofibers during endotoxemia.Biochim Biophys Acta Mol Basis Dis. 2019 Oct 1;1865(10):2765-
2773.
172. Wellmann M, Álvarez-Ferradas C, Maturana CJ, Sáez JC, Bonansco C. Astroglial Ca2+ hyperactivity
requires P2Y1 purinergic receptors and pannexin-1 channel activation in a chronic model of epilepsy.
Frontiers Cell Neurosci. 2018, Oct 11;9:1414.
171. Sáez JC, Contreras-Duarte S, Gómez GI, Labra VC, Santibañez C.A, Gajardo-Gómez R., Avendaño
B.C., Díaz E.F., Montero T.D., Velarde V. Orellana JA. Connexin 43 hemichannel activity promoted by proInflammatory cytokines and high glucose alters endothelial cell function. Front Immunol. 2018 Aug
15;9:1899.
170. Güiza J, Barría I, Sáez JC, Vega JL. Innexins: Expression, Regulation and Functions. Front Physiol
2018 Oct 11;9:1414.
169. Sáez PJ, Sáez JC., Lennon-Duménil A.-M., Vargas P. Role of calcium permeable channels in dendritic
cell migration. Current Opinion Immunol. ;52:74-80 (2018).
168. Gómez GI, Fernández P, Velarde V, Sáez JC. Angiotensin II-Induced mesangial cell damage Is
preceded by cell membrane permeabilization Due to Upregulation of Non-Selective Channels.Int J Mol Sci.
2018 Mar 23;19(4).
167. Sáez PJ, Vargas,P, Shoji KF, Harcha PA, Lennon-Duménil A-M, Sáez JC. ATP promotes the fast
migration of dendritic cells through the activity of pannexin 1 channels and P2X7 receptors. Science
Signaling 2017 Nov 21;10(506). pii: eaah7107. doi: 10.1126/scisignal.aah7107.
166. Barría I., Güiza J., Cifuentes, F.; Zamorano, P.; Sáez, J.C.; González, J.; Vega, J.L. Trypanosoma cruzi
infection induces pannexin-1 channel opening in cardiac myocytes. Amer J Tropical Med & Hygiene 2017
Nov 6. doi: 10.4269/ajtmh.17-0293. [Epub ahead of print]
165. Giaume C, Sáez JC, Song W, Leybaert L, Naus CC. Connexins and pannexins in Alzheimer's disease.
Neurosci Lett. 2017 Sep 8. pii: S0304-3940(17)30740-1.
164. Rovegno M, Sáez JC.Role of astrocyte connexin hemichannels in cortical spreading depression.Biochim
Biophys Acta-Biomembranes. 2017 Aug 29. pii: S0005-2736(17)30264-X.
163. Yi C, Ezan P, Fernández P, Schmitt J, Sáez JC, Giaume C, Koulakoff A. Inhibition of glial
hemichannels by boldine treatment reduces neuronal suffering in a murine model of Alzheimer's disease.
Glia. 65(10):1607-1625 (2017).
162. Charvériat M, Naus CC, Leybaert L, Sáez JC, Giaume C. Connexin-Dependent Neuroglial Networking
as a New Therapeutic Target. Front Cell Neurosci. 2017 Jun 26;11:174.
161. Plotkin LI, Davis HM, Cisterna BA, Sáez JC. Connexins and Pannexins in Bone and Skeletal Muscle.
Curr Osteoporos Rep. 15(4):326-334 (2017).
160. Balboa E, Saavedra F, Cea LA, Vargas AA, Ramírez V, Escamilla R, Sáez JC, Regueira T.
Sepsis-Induced Channelopathy in Skeletal Muscles is Associated with Expression of Non-Selective Channels.
Shock. 49(2):221-228. (2018).
159. Vargas AA, Cisterna BA, Saavedra-Leiva F, Urrutia C, Cea LA, Vielma AH, Gutierrez-Maldonado SE,
Martin AJ, Pareja-Barrueto C, Escalona Y, Schmachtenberg O, Lagos CF, Pérez-Acle T, Sáez JC. On
Biophysical Properties and Sensitivity to Gap Junction Blockers of Connexin 39 Hemichannels Expressed in
HeLa Cells. Front Physiol. 2017 Feb 9;8:38.
158. Sáez JC. Unravelling a novel mechanism for the up-regulation of connexin43 gap junctions between
cells derived from the blood-brain barrier.J Physiol. 595(8):2411-2412 (2017).
157. Puebla C, Retamal MA, Acuña R, Sáez JC. Regulation of connexin-based channels by fatty acids. Front
Physiol. 2017 Jan 24;8:11.
156. Gajardo-Gómez R, Labra VC, Maturana CJ, Shoji KF, Santibañez CA, Sáez JC, Giaume C, Orellana JA.
Cannabinoids prevent the amyloid β-induced activation of astroglial hemichannels: A neuroprotective
mechanism. Glia. 65(1):122-137 (2017).
155. Johnson RG, Le HC, Evenson K, Loberg SW, Myslajek TM, Prabhu A, Manley AM, O'Shea C,
Grunenwald H, Haddican M, Fitzgerald PM, Robinson T, Cisterna BA, Sáez JC, Liu TF, Laird DW, Sheridan
JD.Connexin Hemichannels: Methods for Dye Uptake and Leakage. J Membr Biol. 249(6):713-741 (2016).
154. Cisterna BA, Vargas AA, Puebla C, Sáez JC. Connexin hemichannels explain the ionic imbalance and
lead to atrophy in denervated skeletal muscles. Biochim Biophys Acta-Molecular Basis of Diseases.
1862(11):2168-2176 (2016).
153. Cea LA, Balboa E, Puebla C, Vargas AA, Cisterna BA, Escamilla R, Regueira T, Sáez JC.
Dexamethasone-induced muscular atrophy is mediated by functional expression of connexin-based
hemichannels. Biochim Biophys Acta- Molecular Basis of Diseases. 1862(10):1891-1899 (2016).
152. Maturana CJ, Aguirre A, Sáez JC. High glucocorticoid levels during gestation activate the
inflammasome in hippocampal oligodendrocytes of the offspring. Dev Neurobiol. 77(5):625-642 (2017).
151. Cea LA, Bevilacqua JA, Arriagada C, Cárdenas AM, Bigot A, Mouly V, Sáez JC, Caviedes P. The
absence of dysferlin induces the expression of functional connexin-based hemichannels in human myotubes.
BMC Cell Biol. 2016 May 24;17 Suppl 1:15. doi: 10.1186/s12860-016-0096-6
150. Puebla C, Cisterna BA, Salas DP, Delgado-López F, Lampe PD, Sáez JC. Linoleic acid permeabilizes
gastric epithelial cells by increasing connexin 43 levels in the cell membrane via a GPR40- and Aktdependent mechanism. Biochim Biophys Acta-Cell and Molecular Biology of Lipids. 1861(5):439-448.
(2016).
149. Cea LA, Puebla C, Cisterna BA, Escamilla R, Vargas AA, Frank M, Martínez-Montero P, Prior C,
Molano J, Esteban-Rodríguez I, Pascual I, Gallano P, Lorenzo G, Pian H, Barrio LC, Willecke K, Sáez JC.
Fast skeletal myofibers of mdx mouse, model of Duchenne muscular dystrophy, express connexin
hemichannels that lead to apoptosis. Cell Mol Life Sci. 73(13): 2583-2599 (2016).
148. Graham ZA, Collier L, Peng Y, Saéz JC, Bauman WA, Qin W, Cardozo CP. A Soluble Activin
Receptor IIB Fails to Prevent Muscle Atrophy in a Mouse Model of Spinal Cord Injury. J Neurotrauma.
33(12):1128-1135 (2016).
147. Talaverón R, Fernández P, Escamilla R, Pastor AM, Matarredona ER, Sáez JC.
Neural progenitor cells isolated from the subventricular zone present hemichannel activity and form
functional gap junctions with glial cells. Front Cell Neurosci. Oct 13;9:411. doi: 10.3389/ fncel.2015.00411
(2015).
146. Harcha PA, Vargas A, Yi C, Koulakoff AA, Giaume C, Sáez JC. Hemichannels Are Required for
Amyloid β-Peptide-Induced Degranulation and Are Activated in Brain Mast Cells of APPswe/PS1dE9 Mice.
J Neurosci. 35(25):9526-9538 (2015).
145. Sáez JC, Cisterna BA, Vargas A, Cardozo CP. Regulation of pannexin and connexin channels and their
functional role in skeletal muscles. Cellular and Molecular Life Sciences. 72(15):2929-2935 (2015).
144. Orellana JA, Moraga-Amaro R, Díaz-Galarce R, Rojas S, Maturana CJ, Stehberg J, Sáez JC. Restraint
stress increases hemichannel activity in hippocampal glial cells and neurons. Front Cell Neurosci. 2015 Apr
2;9:102. doi: 10.3389/fncel.2015.00102. eCollection 2015.
143..Riquelme MA, Cea LC, Vega JL, Puebla C,Vargas AA,.Shoji KF, Subiabre M, Sáez JC. Pannexin
channels mediate the acquisition of myogenic commitment in C2C12 reserve cells promoted by P2 receptor
activation. Frontiers in Cell Dev Biol 06 May 2015.
142. Salas D, Puebla C, Lampe PD, Lavandero S, Sáez JC.Role of Akt and Ca2+ on cell permeabilization via
connexin43 hemichannels induced by metabolic inhibition. Biochim Biophys Acta- Molecular Basis of
Diseases. 2015 Mar 14. pii: S0925-4439(15)00068-X.
141. García I. , Maripillán J, Jara O, Ceriani R, Palacios-Muñoz A, Ramachandran J, Olivero P, Pérez-Acle
T, González C, Sáez JC, Contreras JE, Martínez AD. Keratitis-Ichthyosis-Deafness syndrome-associated
Cx26 mutants produce non-functional gap junctions but hyperactive hemichannels when co-expressed with
wild type Cx43. The Journal of Investigative Dermatology Jan 27.
140. Rovegno M, Soto PA, Sáez PJ, Naus C, Sáez JC, von Bernhardi R. ATP released via Connexin 43
hemichannels and P2 receptors mediate secondary cellular damage spread from the trauma zone to distal
zones in astrocyte monolayers. Glia 63(7):1185-1199 2015.
139. Cisterna BA, Cardozo C, Sáez JC. Neuronal involvement in muscular atrophy. Frontiers in Cell.
Neurosc. 2014 Dec 10;8:405. doi: 10.3389/fncel.2014.00405. eCollection 2014..
138. Ardiles AO, Flores-Muñoz C, Toro-Ayala G, Cárdenas AM, Palacios AG, Muñoz P, Sáez JC,
Fuenzalida M, Martínez AD. Pannexin 1 regulates the bidirectional hippocampal synaptic plasticity in the
adult mice. Frontiers in Cell. Neurosci. 2014 Oct 15;8:326.
137. Figueroa V, Retamal MA, Cea LA, Salas JD, Vargas AA, Verdugo CA, Jara O, Martínez AD, Sáez JC.
Gentamicin reversible blocks connexin hemichannels and does not affect gap junction channels. Frontiers
Cell. Neurosci. 2014 Sep 4;8:265.
136. Sáez P., Shoji KF, Aguirre A, Sáez JC. Regulation of hemichannels and gap junction channels by
cytokines in antigen presenting cells. Mediators of Inflammation 2014:742734.
135. Le HT, Sin WC, Lozinsky S, Bechberger J, Vega JL, Guo XQ, Sáez JC, Naus CC. Gap junction
intercellular communication mediated by connexin43 in astrocytes is essential for their resistance to oxidative
stress. J Biol Chem. 289:1345-1354 (2014).
134. Momboisse F, Olivares MJ, Baéz X, Guerra MJ, Flores-Muñoz C, Sáez JC, Martínez AD, Cárdenas
AM. Pannexin 1 channels: new actors in the regulation of catecholamine release from adrenal chromaffin
cells. Frontiers Cell. Neurosci. 2014 Sep 4;8:270.
133. Araya-Secchi R, Kang S-G, Huynh T, Bernardin A, Martinez AD, Sáez JC, Pérez-Acle T, Zhou R.
Characterization of a novel water pocket inside the human Cx26 hemi-channel structure. Biophys. J.
107:599-612.(2014).
132. Retamal MA, Alcayaga J, Bultynck G, Leybaert L, Sáez PJ, Fernández R, LeónLE, Sáez JC. The
opening of connexin and pannexin hemichannels increases the excitability of nodose ganglion sensory
neurons. Frontiers in Cellular Neurosci. Jun 20;8:158.
131. Cea LC, Riquelme MA, Vargas AA, Urrutia C, Sáez JC. Pannexin 1 channels in skeletal muscles.
Frontier in Membr. Physiol. Biophys. Apr 11;5:139.
130. Sáez JC, Leybaert L. Hunting for connexin hemichannels. FEBS Letters 588: 1205-1211 (2014).
129. Shoji KF, Sáez PJ, Harcha PA, Aguila HL, Sáez JC. Pannexin1 channels act downstream of P2X7
receptors in ATP-induced murine T cell death. Channels 8: 1-15 2014.
128. Hernández-Salinas R, Vielma AZ, Arismendi MN, Boric MP, Sáez JC, Velarde V. Boldine prevents
renal alterations in diabetic rats. J Diabetes Res. 2013;2013:593672.
127. Orellana JA, Sáez JC, Bennett MVL, Berman JW, Morgello S, Eugenin EA.HIV increases the release of
dickkopf-1 (DKK1) protein from human astrocytes by a Cx43 hemichannel-dependent mechanism. J.
Neurochem. 128(5):752-763 (2014).
126. Giaume C, Leybaert L, Naus CC, Sáez JC. Connexin and pannexin hemichannels in brain glial cells:
properties, pharmacology and roles. Frontiers Pharmacol. 2013 Jul 17;4:88.
125. Cea LC, Cisterna BA, Puebla C, Frank M, Figueroa XF, Cardozo C, Willecke K,Latorre R, Sáez JC. De
novo expression of connexin hemichannels in denervated fast skeletal muscles leads to atrophy. Proc. Natl.
Acad. Sci. USA 110:16229-16234 (2013). (recommended in F1000Prime).
124. Johnson RG, Sáez JC. We've Had Important Advances in the Connexin/Pannexin Field, Yet There Is
Still Much to Do. Neuropharmacology. 2013 Aug 19. doi:pii: S0028-3908(13)00368-7.
123. Vega JL, Subiabre M, Figueroa F, Schalper KA, Osorio L, González Sáez JC. Role of gap junctions and
hemichannels in parasitic infections. BioMed Res Int. 2013;2013:589130. doi: 10.1155/2013/589130.
122. Aguirre A, Maturana CJ, Harcha PA, Sáez JC. Possible Involvement of TLRs and Hemichannels in
Stress-Induced CNS Dysfunction via Mastocytes, and Glia Activation. Mediators Inflamm.
2013;2013:893521.
121. Sáez PJ, Orellana JA, Vega-Riveros N, Figueroa VA, Hernández DE, Castro JF, Klein AD, Jiang JX,
Zanlungo S, Sáez JC. Disruption in Connexin-based Communication is Associated with Intracellular Ca2+
Signal Alterations in Astrocytes from Niemann-Pick type C Mice. PLOS One 2013 Aug 15;8(8):e71361.
120. Kozoriz MG, Lai S, Vega JL, Sáez JC, Sin WC, Bechberger JF, Naus CC. Cerebral ischemic injury is
enhanced in a model of oculodentodigital dysplasia. Neuropharmacology 2013 May 30.
119. Sáez PJ, Shoji KF, Retamal MA, Harcha PA, Ramírez G, Jiang JX, von Bernhardi R, Sáez JC. ATP is
required and advances cytokine-induced gap junction formation in microglia in vitro. Mediators of
Inflammation 2013;2013:216402.
118. Figueroa XF, Lillo MA, Gaete PS, Riquelme M, Sáez JC. Diffusion of nitric oxide across cell
membranes of the vascular wall requires specific connexin-based channels. Neuropharmacology 75:471-478
(2013).
117. Riquelme MA, Cea LA, Vega JL, Boric MP, Monyer H, Bennett MV, Frank M, Willecke K, Sáez JC..
The extracellular ATP required for potentiation of the adult skeletal muscle contraction is released through
pannexin 1 based channels. Neuropharmacology 75:594-603 (2013).
116. Orellana JA, Williams DW, Sáez JC, Berman JW, Eugenín EA.Pannexin1 hemichannels are critical for
HIV infection of human primary CD4+ T lymphocytes. J. Leuk. Biol. 94(3):399-407 (2013).
115. Aguirre A, Shoji KF, Sáez JC, Henríquez M, Quest AFG. FasL-triggered death of Jurkat cells requires
caspase 8-induced, ATP-dependent cross-talk between Fas and P2X7 receptors. J. Cell Physiol. 228: 485-493
(2013).
114. Bennett MV, Garré JM, Orellana JA, Bukauskas FF, Nedergaard M, Sáez JC. Connexin and pannexin
hemichannels in inflammatory responses of glia and neurons. Brain Res. 1487:3-15 (2012).
113. Fiori MC, Figueroa V, Zoghbi, M.E, Saéz JC, Reuss L, Altenberg GA. Calcium transport through
purified connexin 26 hemichannels. J. Biol. Chem. 287:40826-40834 (2012).
112. Figueroa V, Sáez PJ, Salas JD, Jara O, Martínez AD, Sáez JC. Retamal MA. Linoleic acid induces
opening of connexin26 hemichannels through a PI3K/Akt/Ca2+
-dependent pathway. BBA-Biomembranes
1828:1169-1179 (2012).
111. Cea LA, Riquelme MA, Cisterna B, Puebla C, Vega JL, Rovegno M, Sáez J.C Connexin- and pannexinbased channels in normal skeletal muscles and their possible role in muscle atrophy. J. Membr. Biol. 245:
423-436 (2012).
110. Jara, O., Acuña, R., García, I., Miripillán, J., Figueroa, V., Sáez, JC. Araya R. Lagos, C. Pérez-Acle T,
Berthoud VM, Beyer EC, Martínez AD. Critical role of the first transmembrane domain of Cx26 in regulating
oligomerization and function. Mol. Biol. Cell 23: 3299-3311 (2012).
109. Stehberg J, Moraga-Amaro R, Salazar C, Becerra A, Echeverría C, Orellana JA, Bultynck G, Leybaert L,
Simon F, Sáez JC, Retamal MA. Release of gliotransmitters through astroglial connexin 43 hemichannels is
necessary for fear memory consolidation in the basolateral amygdala. FASEB J. 26: 3649-3657 (2012).
108. Pérez-Armendaris EM, Esparza-Aguilar, Coronel-Cruz C, Cruz-Miguel L, Pinzon-Estrada E, RacañoCamacho E, Zacarias-Climaco G, Fernández P, Espinosa AM, Becker I, Sáez JC, Berumen J, Pérez-Palacios
G. Connexins 26, 32 and 36 are expressed in a cell-specific manner at the end of the secondary transition of
fetal mouse pancreas development and increase during fetal and perinatal life. Anat. Rec. (Hoboken)
295:980-990 (2012).
107. Orellana JA, Sánchez HA, Schalper KA, Figueroa V, Sáez JC. Regulation of intercellular calcium
signaling through calcium interactions with connexin-based channels. Adv. Exp. Med. Biol. 740:777-794
(2012).
106. Eugenín EA, Basilio D., Sáez JC, Orellana JA, Raine CS, Bukauskas F, Bennett MVL, Berman JW. The
role of gap junction channels during physiologic and pathologic conditions of the human nervous system. J.
Neuroimmune Pharmacol. 7:499-518 (2012).
105. Orellana JA, von Bernhardi R, Giaume C, Sáez JC. Glial hemichannels and their involvement in aging
and neurodegenerative diseases. Rev. Neurosci. 23:163-177 (2012).
104. Schalper KA, Riquelme MA, Brañes MC, Martínez AD, Vega JL, Berthoud VM, Bennett MV, Sáez JC.
Modulation of gap junction channels and hemichannels by growth factors. Mol. Biosyst. 8:685-698 (2012).
103. Giaume C, Orellana JA, Abudara V, Sáez JC. Connexin-based channels in astrocytes: how to study their
properties. Methods Mol. Biol. 814:283-303 (2012).
102. Koulakoff A, Mei X, Orellana, JA, Sáez JC, Giaume C. Glial connexin expression and function in the
context of Alzheimer's disease. BBA – Biomembranes 1818(8):2048-57 (2012).
101. Rovegno M, Soto PA, Sáez JC, von Bernhardi R. Biological mechanisms involved in the spread of
traumatic brain damage. Med Intensiva. 36:37-44 (2012).
100. Orellana JA, Sáez PJ, Cortés-Campos C, Elizondo RJ, Shoji KF., Contreras-Duarte Susana, Figueroa V.,
Velarde V, Jiang JX,. Nualart F, Sáez JC, García MA. Glucose increases intracellular free Ca2+ in tanycytes
via ATP released through connexin 43 hemichannels. Glia 60:53-68 (2012).
99. Orellana JA, DíazE,Schalper,KA, Aníbal A. Vargas, Michael VL. Bennett, Sáez J.C. Cation permeation
through connexin 43 hemichannels is cooperative, competitive and saturable with parameters depending on
the permeant species. Biochem. Biophys. Res. Comm. 409:603-609 (2011).
98. Orellana JA, Shoji KF, Abudara V, Ezan P, Amigou E, Sáez PJ, Jiang JX, Naus CC, Sáez JC, Giaume, C.
(2011) Amyloid β-induced death in neurons involves glial and neuronal hemichannels. J Neurosci. 31:4962-
4977.
97. Orellana JA, Froger N, Ezan P, Jiang JX, Bennett MV, Naus CC, Giaume C, Sáez JC.
ATP and glutamate hemichannels mediate neuronal death through released via astroglial connexin 43
activation of pannexin 1 hemichannels. J Neurochem. 118:826-40 (2011).
96. Orellana JA, Figueroa XF, Sánchez HA, Contreras-Duarte S, Velarde V, Sáez JC. Hemichannels in the
Neurovascular Unit and White Matter Under Normal and Inflamed Conditions. CNS Neurol Disord Drug
Targets. 10:404-414 (2011).
95. Garré JM, Retamal MA, Cassina P., Barbeito L, Bukauskas FF, Sáez JC, Bennett, MVL, Abudara V. FGF
-1 induces ATP release from spinal astrocytes in culture and opens pannexin and connexin hemichannels.
Proc. Natl. Acad. Sci. USA 107:22659-22664 (2010).
94 Schalper KA, Sánchez HA, Lee SC, Altenberg GA, Nathanson MH and Sáez JC (2010). Connexin 43
hemichannels mediate the Ca2+ influx induced by extracellular alkalinization. Am. J. Physiol. Cell Physiol
299:C1504–C1515.
93. Sáez JC, Schalper KA, Retamal MA, Orellana JA, Shoji KF, and Bennett MVL. Cell membrane
permeabilization via connexin-hemichannels in living and dying cells. Exp. Cell Res. 316: 2377-2389 (2010).
92. Froger N, Orellana JA, Amigou E, Kozoriz MG., Naus CC, Sáez JC, Giaume C.Inhibition of Cx43
hemichannels activated by pro-inflammatory treatment exhibited a neuroprotective effect against NMDA
excitotocixity. Mol. Cell. Neurosci. 45:37-46 (2010).
91. Orellana JA, Hernández DE, Ezan P, Velarde V, Bennett MVL, Giaume C, Sáez JC. Hypoxia in high
glucose followed by reoxygenation in normal glucose reduces the viability of cortical astrocytes through
increased permeability of connexin 43 hemichannels. Glia 58:329-343 (2010).
90. Froger N., Orellana JA, Ezan P, Amigou E, Sáez, JC, Giaume C. Cannabinoids prevent the opposite
regulation of astroglial connexin43 hemichannels and gap junction channels induced by pro-inflammatory
treatments. J. Neurochem. 111:1383-1397 (2009).
89. Buvinic S, Almarza G, Bustamante M, Casas M, López J, Riquelme M, Sáez JC, Huidobro-Toro JP,
Jaimovich E. ATP released by electrical stimuli elicits calcium transients and gene expression in skeletal
muscle. J. Biol. Chem. 284:34490-34505 (2009).
88. Sánchez HA, Orellana JA., Verselis VK. and Sáez JC. Metabolic inhibition increases the activity of
connexin-32 hemichannels which are permeable to Ca2+
. Am. J. Physiol. 297:C665-78 (2009).
87. Elgueta R, Tobar JA, Shoji KF, De Calisto J, Kalergis AM, Bono MR, Rosemblatt M, Sáez JC. Gap
junctions at the dendritic cell-T cell Interface are key elements for antigen-dependent T cells activation. J.
Immunol. 183:277-284 (2009).
86. Schalper KA, Orellana JA, Berthoud VM, Sáez JC. Dysfunctions of the diffusional membrane pathways
mediated by hemichannels in inherited and acquired human diseases. Current Opinion in Pharmacology
7:486-505 (2009).
85. Orellana JA, Sáez PJ, Shoji KF, Schalper KA, Palacios-Prado N, Velarde V, Giaume C, Bennett MVL,
Sáez JC. Modulation of brain hemichannels and gap junction channels by pro-inflammatory agents and their
possible role in neuro-degeneration. Antioxidants & Redox Signaling 11: 369-99 (2009).
84. Véliz LP, González FG, Duling BR, Sáez JC, Boric MP. Functional role of gap junctions in cytokineinduced leukocyte adhesion and transmigration in vivo. Am. J. Physiol. 295:H1056-H1066 (2008).
83. Schalper KA, Palacios-Prado N. Orellana JA, Sáez JC. Currently used methods for identification and
characterization of hemichannels. Cell Commun Adhes. 15:207-218 (2008).
82. Schalper KA, Palacios-Prado N, Retamal MA, Shoji KF, Martínez AD, Sáez.JC. The connexin
hemichannel composition determines the FGF-1-induced membrane permeability and free Ca2+i responses.
Mol. Biol. Cell. 19: 3501–3513 (2008).
81. Sáez JC. Astrocytes as connexin-dependent signaling cells for local blood flow regulation. Am J Physiol.
294:H586-H587 (2008).
80. Retamal MA,Froger N, Palacios-Prado N, Ezan P,Sáez PJ, Sáez, JC, Giaume, C. Cx43 hemichannels and
gap junction channels in astrocytes are oppositely regulated by pro-inflammatory cytokines released from
activated microglia. J. Neurosci. 27: 13781-13792 (2007).
79. Eugenín EA, González HE, Sánchez HA, Brañes MC, Sáez JC. Inflammatory conditions induce gap
junctional communication between rat Kupffer cells both in vivo and in vitro. Cell. Immunol. 247:103-110
(2007).
78. Retamal MA, Schalper KA, Shoji KJ, Orellana JA, Bennett MVL, Sáez JC. Possible involvement of
different connexin43 domains in plasma membrane permeabilization induced by ischemia-reperfusion. J.
Membr. Biol. 218:49-63 (2007).
77. Retamal MA, Schalper KA, Shoji KF, Bukauskas FF, Bennett MVL, Sáez JC. Opening of connexin 43
hemichannels is increased by lowering intracellular redox potential. Proc. Natl. Acad. Sci. USA 104: 8322-
8327 (2007).
76. Mendoza-Naranjo A, Sáez PJ, Johansson CC, Ramírez M, Mandaković D, Pereda C, López MN,
Kiessling R, Sáez JC, Salazar-Onfray F. Functional gap junctions facilitate melanoma antigen transfer and
cross-presentation between human dendritic cells. J. Immunol. 178: 6949-6957 (2007).
75. Corvalán L, Araya R, Brañes MC, Sáez PJ, Kalergis AM. Tobar, J.A., Theis, M. Willecke K, Sáez, J.C.
Injury of skeletal muscle and specific cytokines induce the expression of gap junction channels in mouse
dendritic cells. J. Cell. Physiol. 211: 649-660 (2007).
74. Orellana JA, Palacios-Prado N, Sáez JC. Chlorpromazine reduces the intercellular communication via gap
junctions in mammalian cells. Toxicol. Appl. Pharmacol. 213: 187-197 (2006).
73. Retamal MA, Cortés CJ, Reuss L, Bennett MVL, Sáez JC. S-nitrosylation and permeation through
connexin 43 hemichannels in astrocytes: induction by oxidant stress and reversal by reducing agents. Proc.
Natl. Acad. Sci. USA. 103: 4475-4480 (2006).
72. Sáez JC, Retamal MA, Basilio DA. Bukauskas FF, Bennett MVL. Connexin-based gap junction
hemichannels: gating mechanisms. Biochim. Biophys. Acta (biomembranes) 1711: 215-224 (2005).
71. Araya R, Eckardt D, Maxeiner S, Krüger O, Theis M, Willecke K, Sáez JC. Expression of connexins
during differentiation and regeneration of skeletal muscle: Functional relevance of connexin43. J. Cell Sci.
118: 27-37 (2005).
70. Contreras JE, Sánchez H, Véliz L, Bukauskas FF, Bennett MVL, Sáez JC. Role of connexin-based gap
junction channels and hemichannels in ischemia-induced cell death in nervous tissue. Brain Res. Rev. 47:
290-303 (2004).
69. Figueroa XF, Alviña K, Martínez AD, Garcés G. Rosemblat, M, Boric M P, Sáez JC. Histamine reduces
gap junctional communication of human tonsil high endothelial cells in culture. Microvasc. Res. 68: 247-257
(2004).
68. Araya R, Riquelme M, Brandan E, Sáez JC. The formation of skeletal muscle myotubes requires
functional membrane receptors activated by extracellular ATP. Brain Res. Rev. 47: 174-178 (2004).
67. Bennett MVL, Contreras JE, Bukauskas FF, Sáez JC. New roles for astrocytes: gap junction
hemichannels have something to communicate. Trends in Neurosc. (TINS) 11: 610-617 (2003).
66. Sáez JC, Berthoud VM, Brañes MC, Martínez AD, Beyer EC. Plasma membrane channels formed by
connexins: Their regulation, and function. Physiol. Rev. 83: 1359-1400 (2003).
65. Sáez JC, Contreras J, Bukauskas F, Retamal M, Bennett MVL. Gap junction hemichannels in astrocytes
of the CNS. Acta Physiol. Scand. 179: 9-22 (2003).
64. Araya R, Eckardt D, Riquelme M, Willecke K, Sáez JC. Presence and importance of connexin43 during
myogenesis. Cell Comm. Adh. 10: 451-456 (2003).
63. Contreras JE, Sáez JC, Bukauskas FF, Bennett MVL. Functioning of Cx43 hemichannels demonstrated
by single channel properties. Cell Comm. Adh. 10: 245-249 (2003).
62. Contreras JE, Sáez JC, Bukauskas FF. and Bennett MVL. Gating and regulation of connexin 43 (Cx43)
hemichannels. Proc. Natl. Acad. Sci. U.S.A. 100: 11388-11393 (2003).
61. Pérez-Armendariz EM, Sáez JC, Bravo-Moreno JF, López-Olmos V, Enders, GC, Villalpando, I.
Connexin43 is expressed in mouse fetal ovary. Anat. Rec. 271A: 360-367, 2003.
60. Eugenín EA, Brañes MC, Berman JW, Sáez, JC. TNF-alpha plus IFN-gamma induce connexin43
expression and formation of gap junctions between human monocytes/macrophages that enhance
physiological responses. J. Immunol. 170(3):1320-1328, (2003).
59. Contreras JE, Sánchez HA, Eugenín EA, Speidel D, Theis M, Willecke K, Bukauskas FF, Bennett MV,
Sáez JC. Metabolic inhibition induces opening of unapposed connexin 43 gap junction hemichannels and
reduces gap junctional communication in cortical astrocytes in culture. Proc. Natl. Acad. Sci. U. S. A. 99:
495-500 (2002).
58. González HE, Eugenín EA, Garcés G, Solis N, Pizarro M, Accatino L, Sáez JC. Regulation of hepatic
connexins in cholestasis: possible involvement of Kupffer cells and inflammatory mediators. Amer. J.
Physiol. 282: G991-G1001 (2002).
57. Brañes MC, Contreras JE, Sáez JC. Activation of human polymorphonuclear cells induces formation of
functional gap junctions and expression of connexins. Med. Sci. Monit, 8: BR313-BR323, (2002).
56. Martínez AD, Eugenín EA, Brañes MC, Bennett MVL, Sáez JC. Identification of second messengers that
induce expression of functional gap junctions in microglia cultured from newborn rats. Brain Res. 943: 191-201
(2002).
55. Eugenín EA, Eckardt D, Theis M, Willecke K, Bennett MVL, Sáez JC. Microglia at stab wound express
connexin43 and in vitro form functional gap junctions after treatment with interferon-gamma and tumor necrosis
factor. Proc. Natl. Acad. Sci. U.S.A. 98: 4190-4195 (2001).
54. Bravo-Moreno JF, Díaz-Sánchez V, Montoya-Flores JG, Lamoyi E, Sáez JC, Pérez-Armandariz M. Expression
of connexin 43 in mouse Leydig, Sertoli and germinal cells at different stages of postnatal development. Anat. Rec.
264: 13-24 (2001).
53. Martínez AD Sáez JC. Regulation of astrocyte gap junctions by hypoxia-reoxygenation. Brain Res. Rev. 32:
250-258 (2000).
52. Hetzberg EL, Sáez JC, Corpina RA, Roy C, Kessler JA. Use of antibodies in the analysis of connexin43
turnover and phosphorylation. Methods 20: 129-139 (2000).
51. Sáez JC, Corvalán LA, Eugenín EA, González H, Martínez A, Palisson F. Gap junctions in cells of the immune
system: structure, regulation and possible functional roles. Brazilian J. Med. Biol. Res. 33: 447- 455 (2000).
50. Berthoud VM, Hall DH, Strahsburger E, Beyer EC and Sáez JC. Gap junctions in the chicken pineal gland.
Brain Res. 861: 257-270 (2000).
49. Martínez AD, Sáez JC. (1999) Arachidonic acid-induced dye uncoupling in rat cortical astrocytes is mediated
by arachidonic acid byproducts. Brain Res. 816:411-423.
48. Abudara V, Garcés G, Sáez JC. Cells of the carotid body express connexin43 which is up-regulated by cAMP.
Brain Res. 849: 25-33 (1999).
47. González CA, Garcés G, Sáez JC, Schobitz K, Rodriguez EM. The ependymocytes of the bovine
subcommissural organ are functionally coupled though gap junctions. Neurosc. Lett. 262: 175-178 (1999).
46. Sáez, J.C., Martínez, A., Brañes, M.C., González, H. Regulation of gap junctions by protein phosphorylation.
Brazilian J. Med. Biol. Res. 31: 593-600 (1998).
45. Eugenín EA, González H, Sáez CG, Sáez JC. Gap junction communication coordinates vasopressin-induced
glycogenolysis in rat hepatocytes. Amer. J. Physiol. 274: G1109-G1116 (1998).
44. Eugenín EA, Sáez CG, Garcés G, Sáez JC. Regulation of glycogen content in rat pineal gland by epinephrine.
Brain Res. 760: 34-41 (1997).
43. Hermoso M, Sáez JC, Villalón M. Identification of gap junctions in the oviduct and regulation of connexins
during development and by sexual hormones Eur. J. Cell Biol. 74: 1-9 (1997).
42. Sáez JC, Nairn AC, Czernik AJ, Fishmann GI, Spray DC, Hertzberg EL. The functional state of gap junctions
between neonatal rat cardiac myocytes is directly related to changes in the state of phosphorylation of connexin43.
J. Mol. Cell. Cardiol. 29: 2131-2145 (1997).
41. Tan IP, Roy C, Sáez JC. Sáez CG, Paul, D, Risley MS. Regulated assembly of connexin33 and connexin43 into
Sertoli cell gap junctions. Biol. Reproduc. 54: 1300-1310 (1996).
40. Hofer A, Sáez JC, Trosko JE, Spray DC, Dermietzel R. C-erb/neu transfection induces
communication-incompetence via gap junctions in glial cells. J. Neurosc. 16: 4311-4321 (1996).
39. Tanowitz HB, Factor SM, Morris SA, Spray DC, Huang H, Chen B, Campos de Carvalho AC, Sáez JC, Chris,
JC, Weiss LM, Wittner M. Miopathogenesis of Chagas' disease. Basic and Applied Myology. 5: 261-280 (1995).
38. Fallon MB, Nathanson MH, Mennone A, Sáez JC, Anderson JM. Altered expression and function of
hepatocyte gap junctions following common bile duct ligation. Amer. J. Physiol. 268: C1186- C1194 (1995). 37.
Nathanson, M., Burgstahler AD, Mennone A, Fallon MB, González C, Sáez JC. Organization of intercellular Ca2+
waves in rat liver. Amer. J. Physiol. 269: G167-G171 (1995).
36. Jara PI, Boric MP, Sáez JC. Leukocytes express connexin43 after activation with lipopolysaccharide and
appear to form gap junctions with endothelial cells after-ischemia-reperfusion. Proc. Natl. Acad. Sci. USA
92:7011-7015 (1995).
35. Takens-Kwak BR, Sáez JC, Wilders R, Fishman GI, Hertzberg EL, Spray DC, Jongsma H. cGMP-dependent
phosphorylation of connexin43: influence on gap junction channel conductance and kinetics. Pflüger Arch.-Eur. J.
Physiol. 430: 770-778 (1995).
34. Sáez JC, Moreno AP, Spray DC. Norepinephrine induces Ca2+ release from intracellular stores in rat
pinealocytes. J. Pineal Res. 16:-57-61 (1994).
33. Moreno AP, Sáez JC, Fishman GI, Spray DC. Human connexin43 gap junction channels: regulation of unitary
conductances by phosphorylation of the channel protein. Circ. Res. 74: 1050-1057 (1994).
32. Berthoud VM, Sáez JC. Changes in connexin43, the gap junction protein of astrocytes, during development of
the rat pineal gland. J. Pineal Res. 14: 67-72 (1993).
31. Berthoud VM, Rook M, Hertzberg EL, Sáez JC. On the mechanism of cell uncoupling induced by a tumor
promoter phorbol ester in clone 9 cells, a rat liver epithelial cell line. Eur. J. Cell Biol. 62: 384-396 (1993).
30. Berthoud VM, Ledbetter MLS, Hertzberg EL, Sáez JC. Connexin43 in MDCK cells: regulation by a tumor
promoting phorbol ester and Ca2+
. Eur. J. Cell Biol. 57: 40-50 (1992).
29. Risley M, Tan I, Roy C, Sáez JC. Cell, age, and stage-dependent distribution of connexin43 gap junctions in
testes. J. Cell Sci. 103: 81-96 (1992).
28. Berthoud VM, Iwanij V, García AM, Sáez JC. Connexins and glucagon receptors during development of the
hepatic acinus. Amer. J. Physiol. 26: G650-G658 (1992).
27. Sáez JC, Berthoud VM, Kadle R., Nicholson BJ, Bennett MVL, Dermietzel, R. Pinealocytes in rats:connexin
identification and increase in coupling caused by norepinephrine. Brain Res. 568: 265-275 (1991).
26. Bennett MVL, Bargiello TA, Barrio L, Spray DC, Hertzberg EL, Sáez JC. Gap junctions: New Tools, New
Answers, New Questions. Neuron 6: 305-320 (1991).
25. Sáez JC, Spray DC, Hertzberg EL. Gap junctions: Biochemical Properties and Regulation under Physiological
and Toxicological Conditions. In vitro toxicology 3: 69-86 (1990).
24. Sáez JC, Nairn AC, Czernik AJ, Spray DC, Hertzberg EL, Greengard P, Bennett MVL. Phosphorylation of
connexin32, a hepatocyte gap junction protein, by cAMP-dependent protein kinase, protein kinase C and Ca2+/
calmodulin-dependent protein kinase II. Eur. J. Biochem. 192: 263-273 (1990).
23. Sáez JC, Gregory WA, Dermietzel R, Hertzberg E, Watanabe T, Reid L, Bennett MVL, Spray DC. cAMP
delays disappearance of gap junctions between pairs of rat hepatocytes in primary cultures. Amer. J. Physiol. 257:
C1-C11 (1989).
22. Sáez JC, Connor JA, Spray DC, Bennett MVL. Hepatocyte gap junctions are permeable to the second
messengers inositol 1,4,5-trisphosphate and calcium ions. Proc. Natl. Acad. Sci. U.S.A. 86: 2708-2712 (1989).
21. Samuelson AC, Stockert RJ, Novikoff AB, Novikoff PM, Sáez JC., Spray DC, Wolkoff A. Influence of
cytosolic pH on receptor-mediated endocytosis of asialoorosomucoid. Amer. J. Physiol. 254: C829-C838 (1988).
20. Fujita M, Spray DC, Choi H, Sáez JC, Watanabe T, Rosenberg L, Reid L. Glycosaminoglycans and
proteoglycans induce gap junction expression and restore transcription of-tissue-specific mRNAs in primary liver
cultures. Hepatology 7: 15-95 (1987).
19. Spray DC, Fujita M, Sáez JC, Choi H, Hertzberg E, Rosenberg LM, Reid L. Proteoglycans and
glycosaminoglycans induce gap junction synthesis and function in primary liver cultures. J. Cell Biol. 105: 541-551
(1987).
18. Sáez JC, Spray DC, Kessler JA, Bennett MVL. Superoxide dismutase protects cultured neurons against death
by starvation. Proc. Natl. Acad. Sci. U.S.A. 84: 3056-3059 (1987).
17. Sáez JC, Bennett MVL, Spray DC. Carbon tetrachloride at hepatotoxic levels reversibly blocks gap junctional
communication between rat liver cells. Science 236: 967-969 (1987).
16. Sáez JC, Spray DC, Nairn AC, Hertzberg EL, Greengard P, Bennett MVL. cAMP increases junctional
conductance and stimulates phosphorylation of the 27kDa principal gap junction polypeptide. Proc. Natl. Acad.
Sci. U.S.A. 83: 2473-2477 (1986).
15. Spray DC, Sáez JC, Brosius D, Bennett MVL, Hertzberg EL. Isolated liver gap junctions: gating of
transjunctional currents is similar to that in intact pairs of rat hepatocytes. Proc. Natl. Acad. Sci. USA 83:5494-
5497 (1986).
14. Fujita M, Spray DC, Choi H, Sáez JC, Jefferson DM, Hertzberg E, Rosenberg LC, Reid L. Extracellular matrix
regulation of cell-cell communication and tissue-specific gen expression in primary liver cultures. Prog. Clin. Biol.
Res. 226: 333-360 (1986).
13. Sáez JC, Cifuentes F, Ward PH, Günther B, Vivaldi E. Tourniquet shock in rats: effect of allopurinol on
biochemical changes of the gastrocnemius muscle subjected to ischemia followed by reperfusion. Biochem. Med.
Met. Biol. 35: 199-209 (1986).
12. Vivaldi E, Ward PH, Sáez JC, Günther B. Pathogenesis of cell death: point of no return, Part I. Rev. Med.
Chile 113: 238-242 (1985).
11. Vivaldi E, Ward PH, Sáez JC, Günther B. Pathogenesis of cell death: point of no return, Part II. Rev. Med.
Chile 113: 364-368 (1985).
10. Sáez JC, Günther B, Ward PH, Vivaldi E. Superoxide radicals in the pathogenesis of burn shock. Circ. Shock
12: 229-239 (1984).
9. Quevedo L, Melo R, Sáez JC, Cifuentes F. Electrophysiological blocking activity on muscle fibers of lycorine,
an antimitotic alkaloid. Neuropharmacology 23: 391-394 (1984).
8. Kessler JA, Spray DC, Sáez JC, Bennett MVL. Determination of synaptic phenotype: Insulin andcAMP
independently initiate development of electrotonic coupling between cultured sympathetic neurons. Proc. Natl.
Acad. Sci. U.S.A. 81: 6235-6239 (1984).
7. Norris B, Sáez JC, Wrcka R, Concha J. Effect of pentobarbitone in isolated toad skin.
Neuropharmacology 22: 513-518 (1983).
6. Sáez JC, Günther B, Ward PH, Vivaldi E. Etiopathogenesis of burn shock: A new hypothesis. IRCS Med. Sci.
11: 645 (1983).
5. Quevedo L, Aguayo L, Concha J, Cid H, Sáez JC. Electrophysiological effect of methyl-3-0-methyl gallate on
single muscle fibers. Pharmacology 23 5: 293-296 (1981).
4. Sáez JC, Vivaldi E, Günther B. Tourniquet shock in rats: appearance of lactic dehydrogenase isoenzymes in
serum. IRCS Med. Sci. 10: 191-192 (1982).
3. Norris B, Sáez JC, Concha J. Stimulatory effect of pentobarbitone in low concentration on toad skin (Na-K)
ATPase. IRCS Med. Sci. 10: 193-194 (1982).
2. Sáez JC, Norris B, Concha J. Effect of colchicine on the electrophysiologic properties of the toad sciaticgastrocnemius "in vivo". Pharmacology 25: 278-285 (1982).
1. Mayer H, Sáez JC, Herrera R, Concha J. Acute mushroom poisoning. Rev. Med. Chile 108: 671-672 (1980).
BOOKS AND SPECIAL EDITIONS:
1. Sáez JC and Jiang J. (Guest Eds) Special issue on Pannexin- and Connexin-Based Channels under Normal
and Pathological Conditions Int J Mol Sci. 2021.
2. Langlet F, Sáez JC, García MA. (Guets Eds.) Special issue: Involvement of tanycytes in the
neuroendocrine control of energy homeostasis. Frontiers in Endocrinology (2020).
3. Bai D and Sáez JC (Eds) Gap Junction channels and hemichannles. CRC Press | Taylor & Francis Group.
(2017).
4. Sáez JC (Guest Ed.) Special issue II on connexin and pannexin channels BMC Cell Biology 2017.
5. Sáez JC and Martínez AD (Guest Eds) Special issue I on connexin and pannexin channels BMC Cell
Biology 2016.
6. Sáez JC (Guest Ed.) Neuropharmacology. Connexin and pannexin channels. (2013).
7. Sáez J.C. (Guest Ed.) Antioxidant & Redox Signaling. Gap junctions and diseases. (2009).
8. Sáez J.C. (Guets Ed.) and Bennett, M.V.L. (Co-Ed.) Chemical and electrical synapses: recent progress.
Brain Res. Rev. vol. 47 (2004).
9. Latorre R and Sáez JC. (Eds.) From Ion Channels to Cell-to-Cell Conversations. Plenum Press, NY
(1997).
BOOK CHAPTERS AND OTHER PUBLICATIONS (NON-ISI).
44. In: BOOK TITLE & ISBN: "Biology of Trypanosoma cruzi," 978-1-83968-204-9. Book Chapter: Possible Role
of Gap Junction Channels and Non-Junctional Channels in the Infection Caused by Trypanosoma cruzi Vega JL,
Juyumaya C, Rodríguez L, Güiza J, Gutíerrez C, Barría I, Sáez JC. 2019 pp1-12. IntechOpen 10 Lower Thames
Street, London, EC3R 6AF, UK. 2018
43. Gómez, G., Velarde V., and Sáez J.C. Connexin-based channnels and RhoA/ROCK pathway in angiotensin II
induced kidney damage. IntechOpen UK, London.
42. Sáez J.C. Does Inflammatory Response Cause Tissue Dysfunction in Chronic Diseases? (Eds.: Edward M De
Robertis and Marcelo Sánchez Sorondo).pp. 64-72. Librería Editrice Vaticana, Vatican City, 2018.
41. Vega JL, Barría, I, Güiza,J. González,J and Sáez JC. Chapter: Involvement of gap junction proteins in
infectious diseases caused by parasites. Book: Immunology and Microbiology. Natural Remedies in the Fight
Against Parasites. Editors: H. Khater, M. Govindarajan, and G. Benelli, 139-154, In Tech, Rijeka, Croacia. 2017.
40. Sáez, J.C. and Nicholson, B.J. Connexin and Pannexin Based Channels in the Nervous System – Gap Junctions
and more. 3rd edition of From Molecules to Networks (ed. Byrne & James Roberts). Elsevier Science USA. Pp.
257-283(2013)
39. Sáez P.J., Shoji K.F., and Sáez J.C. Gap junctions in antigen-presenting cells. In: Connexin cell communication
channels. Roles in the iummune system and immunopathology.(Eds. Oviedo-Orta, Kwak B.D. and Evans W.H.)
CRC Press Taylor & Francis Group, Boca Raton Fl, USA. 61-88, 2013.
38. Giaume C., Froger N., Orellana J.A., Retamal M.A. and Sáez J.C. Impact of microglial activation on astroglial
connexin expression and function in brain inflammation. In: Connein cell communication channels. Roles in the
iummune system and immunopathology.(Eds. Oviedo-Orta, Kwak B.D. and Evans W.H.) CRC Press Taylor &
Francis Group, Boca Raton Fl, USA. 219-231, 2013.
37. Vega J.L., Baeza E., Berthoud VM and Sáez JC. Connexins as substrates for protein kinases and
phosphoprotein phosphatases. Publicado en: Protein Kinases. Editado por: Gabriela Da Silva Xavier. Publicado por:
InTech Open access publisher. ISBN 978-953-51-0640-1, pp. 1-16, 2012.
36. OrellanaJA, Giaume C and Sáez JC. "Role of Connexin Hemichannels in Neurodegeneration".
Neurodegenerative Diseases - Processes, Prevention, Protection and Monitoring. (ed. Raymond Chuen-Chung
Chang). InTech. 10:235-254, 2011.
35. Giaume C., Orellana J.A., Abudara V. and Sáez J.C. Connexin based channels in astrocytes: how to study their
properties. Methods and protocols. (ed. Richard Milner). Spring Verlag Edition Methods Mol Biol. 2012. 814:283-
303.
34. Sáez, J.C. and Nicholson, B.J. Connexin and Pannexin Based Channels in the Nervous System – Gap Junctions
and more. 2nd edition of From Molecules to Networks (ed. Byrne & James Roberts). Elsevier Science USA. Pp.
447-470 (2009).
33. Schalper K. A. and Sáez, J.C. Dysfunction of transmembrane diffusional transport via connexin-based
hemichannels in human genetic diseases. In:. Ed. Sobrevia, L. Editorial: Research Signpost, Kerala, India, pp. 1-
31(2009).
32. Brañes, M.C., Sáez A., Villalón, M.J. and Sáez, J.C. Uniones en hendidura y su papel funcional en el tracto
reproductor femenino. Rev. Chil. Obstet. Genicol. 69: 60-66 (2004).
31. Jara, P.I., Boric y Sáez, J.C. Uniones intercelulares comunicantes y respuesta inflamatoria. 1: 6-15 (2002)
30. Jara, P.I. y Sáez, J.C. ¿Todas las formas de vida se transmiten a través del DNA o RNA? Clínica y Ciencia 1:
33-38 (2001).
29. Sáez, J. C., Araya, R., Brañes, M.C., Concha, J.M., Contreras, J., Eugenín, E.A., Martínez, A.D., Palisson F.,
and Sepúlveda, M.A. Gap junctions in inflammatory responses: connexins, regulation and possible functional roles.
In: “Gap junctions-Molecular basis of cell communication in health and diseases. Vol. (Peracchia, C., ed.). Of
Current Topic in Membranes. Academic Press. Pp.555-579 (2000).
28. Abudara, V., Eyzaguirre, C. and Sáez, J.C. Regulation of rat carotid body gap junctions by cAMP. Adv. Exp.
Med. Biol. Kluwer Academic/Plenum Publishers vol. 475 pp. 359-369 (2000).
27. Laird, D.W. and Sáez, J.C. Posttranscriptional events in expression of gap junctions. In: Advances in Molecular
Cell Biology. Chapter 3. (Hertzberg, E.L., ed.). Vol. 18, JAI Press, Greenwich, Connecticut. Pp. 99-128 (2000).
26. Sáez, J.C., Sepúlveda, M., Araya, R., Sáez, C.G., and Palisson, F. Concanavalin A-activated lymphocytes form
gap junctions that increase their rate of DNA replication. In: Gap Junctions (Werner, R., ed.) IOS Press, Amterdam,
Netherlands, p. 372-376 (1998).
25. Martínez, A. and Sáez, J.C. Arachidonic acid-induced cell uncoupling is rat astrocytes depends on cyclo- and
lipooxygenase metabolism and is blocked by melatonin. In:Gap Junctions (Werner, R., ed.) IOS Press, Amterdam,
Netherlands, p. 244-248 (1998).
24. Boric, M.P., roth, A., Jara, P. and Sáez, J.C. Gap junction between leukocytes and endothelium: expression of
connexin43 in adherent or activated cells. In: From Ion Channels to cell-to-cell conversation (Latorre, R. and Sáez,
J.C., eds) Plenum Press, New York. p. 249-366 (1997).
23. Sáez, C.G., Eugenín, E., Hertzberg, E.L. and Sáez, J.C. Regulation of gap junctions in rat liver during acute and
chronic CCl4-induced liver injury. In: From Ion Channels to cell-to-cell conversation (Latorre, R. and Sáez, J.C.,
eds) Plenum Press, New York. p. 367-380 (1997).
22. Sáez, J.C., Nairn, A.C., Czernik, A.C., Fishman, G.I., Spray, D.C. and Hertzberg, E.L. Cardiac myocytes gap
junctions: phosphorylation of Cx43 through a protein kinase C-dependent pathway. In: From Ion Channels to cellto-cell conversation (Latorre, R. and Sáez, J.C., eds) Plenum Press, New York. p. 381-394 (1997).
21. Villalón, M., Hermoso, M., Budinich, M., Aguilera, J. and Sáez, J.C. Regulation of smooth muscle activity and
gap junction by sexual hormones in the rat oviduct. In: From Ion Channels to cell-to-cell conversation (Latorre, R.
and Sáez, J.C., eds) Plenum Press, New York. p. 381-387 (1997).
20. Nathanson, M.H., Fallon, M.B., Bugstahler, A.D. Menone, A., Scholsser, S.F., González, C. and Sáez, J.C.
Intercellular calcium signaling in liver. In: From Ion Channels to cell-to-cell conversation (Latorre, R. and Sáez
J.C., eds) Plenum Press, New York. p. 388-396 (1997).
19. Sáez, J.C. Intercellular gap junctional communication is required for optimal metabolic response of the liver.
Hepatology March: 775-776 (1997)
18. Sáez, J.C. Uniones Intercelulares, In: Biofísica y Fisiología Celular (Latorre, R., Bezanilla, F. and López
Barneo, J., eds.) SALVAT, Spain. 407-434 (1996).
17. Sáez, J.C. y Sáez, C.G. Fisiología y fisiopatología de las uniones en hendidura. In: Sindrome de Shock
(Vivaldi, E.C. y Hernandez, G., eds.) Editorial Anibal Pinto, Concepción, Chile. 147-176 (1995).
16. Barrio, L.C., Beyer, E.C. and Sáez, J.C. Comparison of voltage dependence of chick connexin45 and 42
channels expressed in pairs of Xenopus oocytes. In: Progress in Cell Research (Kanno, Y., Katoaka, K., Shiba, Y.,
Shibata, Y. and Shimazu, T., eds.) vol. 4, Elsevier Science B.V. (Amsterdam). pp. 391-394 (1995).
15. Spray, D.C., Rook, M.B., Moreno, A.P., Sáez, J.C., Christ, G.J., Campos de Carvalho, A.C. and Fishman, G.I.
Cardiovascular gap junctions: gating properties, function and dysfunction. In: Ion Channels in the Cardiovascular
system: Function and Dysfunction. (Spooner, P.M., Brown, A.M., Catterall, W.A., Kaczorowski, G.J. and Strauss,
H.C., eds.) Futura Publishing, NY Pp. 185-217 (1994).
14. Spray, D.C., S. Bai, R.D. Burk, and J.C. Sáez. Regulation and function of liver gap junctions and their genes.
Prog Liver Dis 12: 1-18, 1994.
13. Spray, D.C., Sáez, J.C., Hertzberg, E.L. and Dermietzel, R. Gap Junctions in Liver: Composition, Function and
Regulation. In: The Liver: Biology and Pathobiology (Eds, I.M. Arias, W.B. Jakoby, H. Popper, D. Schachter and
D.A. Shafritz), Raven Press Ltd., New York, Thrid edition, 951-967 (1994).
12. Spray, D.C., Bai, S., Burk, R.D. and Sáez, J.C. Regulation and Function of Liver Gap Junctions and their
Genes. In: Progress in Liver Diseases (Boyer, J.L. and Ockner, R.K., eds.) W.B. Saunders Co. vol. 12. pp. 1-18
(1994).
11. Sáez, J.C., V.M. Berthoud, A.P. Moreno and Spray D.C. Gap junctions: Multiplicity of controls in
differentiated and undifferentiated cells and possible functional implications. In: Advances in Second Messenger
and Phosphoprotein Research (Shenolikar, S. and Nairn, A.C., eds.) Vol. 27. Raven Press, New York., pp. 257-262
(1993).
10. Sáez, J.C., Nairn, A.C., Czernik, A.J., Spray, D.C. and Hertzberg, E.L. Rat heart connexin43: regulation by
phosphorylation in heart. Gap Junctions. In: Progress in Cell Research (Hall, J.E., Zampighi, G.A. and Davis, R.M.,
eds.), Elsevier Science Publishers. vol. 39, pp. 263-269 (1993).
9. Berthoud, V.M., Ledbetter, M.L.S., Hertzberg, E.L. and Sáez, J.C. Regulation of gap junctions by cell contact
and phosphorylation in MDCK cells. Gap Junctions. In: Progress in Cell Research (Hall, J.E., Zampighi, G.A. and
Davis, R.M., eds.), vol. 3. Elsevier Science Publishers. pp. 257-262 (1993).
8. Sáez, J.C. and Spray, D.C. Cell Junctions. In: Encyclopedia of Human Biology. Academic Press, Inc. Pp.267-
278 (1991).
7. Sáez, J.C., Bennett, M.V.L. and Spray, D.C. Hepatocyte gap junctions: Metabolic regulation and possible role in
liver metabolism. In: Transduction in Biological Systems. (Hidalgo, C., Bacigalupo, J. Jaimovich, E. and Vergara,
J., eds.) Plenum. Publ. Co., NY. pp. 231-243 (1990).
6. Spray, D.C., Sáez, J.C. and Hertzberg, E.L. Junctions between hepatocytes: Structural and regulatory factors. In:
The Liver: Biology and Pathobiology (Eds, I.M. Arias, W.B. Jakoby, H. Popper, D. Schachter and D.A. Shafritz),
Raven Press Ltd., New York, Second edition, pp. 851-866 (1988).
5. Spray, D.C. and Sáez, J.C. Agents that regulate gap junctional conductance: Sites of action and specificities. In:
Biochemical Regulation of Intercellular Communication. Advances in Modern Environmental Toxicology (Series
Editor, Vol.XIV, M.A.Mehlman) pp. 1-27 (1988).
4. Spray, D.C., Sáez, J.C. Burt, J.M., Wantanabe, T., Reid, L.M., Hertzberg, E.L. and Bennett, M.V.L. Gap
junctional conductance: multiple sites of regulation. In: Gap junctions. (Modern Cell Biology. (Series Editor Vol. 7,
B. Satir) (eds., E.L. Hertzberg and R.G. Johnson) Alan R. Liss., NY, pp.-227-244 (1988).
3. Bennett, M.V.L., Sáez, J.C. and Spray, D.C. Multiplicity of controls of gap junctional communication. Puerto
Rico Health Sci. J. 7, 126-132 (1988)
2. Fujita, M.,Spray, D.C., Choi, H., Sáez, J., Jefferson, D.M., Hertzberg, E., Rosenberg, L.C. and Reid, L.M.
Extracellular matrix regulation of cell-cell communication and tissue specific gene expression in primary liver
cultures. In: Cellular Endocrinology: Hormonal Control of Embryonic and Cellular Differentiation. Prog. Clin.
Biol. Research. Alan R. Liss, Inc. pp. 333-360 (1986).
1. Kessler, J.A., Spray, D.C., Sáez, J.C. and Bennett, M.V.L. Development and regulation of electrotonic coupling
between cultured sympathetic neurons. In: Gap Junctions, (Eds., M.V.L. Bennett and D.C. Spray) Cold Spring
Harbor Laboratory, Cold Spring Harbor, NY, pp. 231-240 (1985).
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