Leila Arabi

Leila Arabi

Pharm.D., Ph.D.

Assistant professor of Pharmaceutical Nanotechnology since February 2017

Department of Pharmaceutical Nanotechnology, School of Pharmacy,

Mashhad University of Medical Sciences, Mashhad, Iran

Email: [email protected], [email protected]

EDUCATION:

Doctor of Philosophy (Ph.D.) in Pharmaceutical Nanotechnology Oct 2009- Jan 2016

School of Pharmacy, Mashhad University of Medical Sciences, Iran.

Doctor of Pharmacy (Pharm.D.)

School of Pharmacy, Mashhad University of Medical Sciences, Iran. Oct 2003- Sep 2009

Visiting Research Scientist

Molecular Pathology department of the Institute for Pathology, Aug 2012-Aug 2013

University Hospital Basel-Switzerland.

RESEARCH INTERESTS AND EXPERIENCE:

Targeted Drug Delivery

Liposomes

Cancer Stem Cells

Targeting Tumor Cells and Microenvironment

No-Viral Vectors for Gene Delivery

Non-coding RNA

Cancer Immunotherapy

-Ph.D. thesis: “in vitro and in vivo targeted drug delivery to cancer stem cells isolated from murine colon carcinoma by doxorubicin nanoliposomes conjugated with monoclonal antibodies”, under supervision of Dr. Mahmoud Reza Jaafari, Dr. Ali Badiee, Dr. Fatemeh Mosaffa. (19.93 out of 20)

-Pharm.D. thesis: “Preparation and characterization of liposmes modified with lipopolymers constructed from polyethyleneimine and bromoalkane derivatives, as a non-viral gene transfer system” under supervision of Dr. Bizhan Malaekeh-nikouee and Dr. Mohammad Ramezani. (19.5 out of 20).

-List of projects in molecular pathology department in University Hospital Basel:

-Molecular regulation of Cancer Stem Cells in gastrointestinal neoplasia.

-Prognostic and therapeutic implications of the oncogenic miR-17-92 cluster in Osteosarcoma.

-Evaluation of putative amplification target genes (Vinculin and KCNMA1) in prostate and breast cancer.

-Establishing a system for testing targeted and personalized medicines in malignant pleural effusions from cancer patients by using the xCelligence system (Roche Applied System)
in collaboration with Roche Company.

-Working on isolation of clonal populations by DNA content-based flow sorting and applying the DNA from the different populations to array comparative genomic hybridization (aCGH) for detection of genomic aberrations.

PUBLICATIONS:

1. Arabi L, Badiee A, Mosaffa F, JaafariMR.”Targeting CD44 expressing cancer cells with anti-CD44 monoclonal antibody improves cellular uptake and antitumor efficacy of liposomal doxorubicin”, J Control Release. 2015 Dec 28;220(Pt A):275-86

2. Quagliata L, Matter M, Piscuoglio S ,Arabi L, Ruiz C, Procino A, Kovac M, Moretti F, Makowska Z, Tornillo L, Diederich S, Heim M.H, CilloC and Terracciano L,”lncRNA HOTTIP/HOXA13 expression is associated with disease progression and predicts clinical outcome in HCC patients”, Hepatology. 2014 Mar;59(3):911-23

3. Ruiz C, Kustermann S, Pietilae E, Vlajnic T, Baschiera B, Arabi L, Lorber T, Oeggerli M, Savic S, Obermann E, Dirnhofer S, Singer T, Rothschild S, Zippelius A, Roth A, Bubendorf L. “Culture and drug profiling of patient derived malignant pleural effusions for personalized cancer medicine”, PLoS One. 2016 Aug 22;11(8)

4. Andreozzi M, Quagliata L, Burmeister K, Arabi L, Schneider S, Tornillo L and Terracciano L, “DNA in situ Hybridizations for VEGFA Gene Locus (6p12) in Human Tumor Tissue”,Bio-Protocol, Aug 2015

5. Ruiz C, Vlajnic T, Arabi L, Wyttenbach S, Baschiera B, Rothschild S, Kustermann S, Zippelius A, Roth A, Bubendorf L, “Abstract 4681: The use of patient derived malignant effusions as in vitro models for a personalized healthcare-approach in anticancer therapy”, Cancer Research, AACR Annual Meeting; April 5-9, 2014; San Diego. Published Oct 2014.

6. Arabi L, Gsponer J, Smida J, Nathrath M, Jundt G, Ruiz C, QuagliataL and BaumhoerD,”Upregulation of the miR-17-92 cluster and its two paraloga in osteosarcoma – reasons and consequences”, Genes Cancer. 2014 Apr;5(1-2):56-63.

7. Yazdani M, Jalali SA, Badiee A, Shariat S, Mansourian M, Arabi L, Abbasi A, Saberi Z, Jaafari MR. "Stimulation of Tumor Specific Immunity by P5 HER-2/NeuGeneratedpeptide Encapsulated in Nano-Liposomes with High Phase Transition Temperature Phospholipids", Curr Drug Deliv. 2016 Jul 12.

8. Alipour Talesh G, Ebrahimi Z, Badiee A, Mansourian M, Attar H, Arabi L, Jalali SA, Jaafari MR."Poly (I:C)-DOTAP cationic nanoliposome containing multi-epitope HER2-derived peptide promotes vaccine-elicited anti-tumor immunity in a murine model", Immunol Lett. 2016 Aug;176:57-64.

9. Moosavian SA, Abnous K, Akhtari J, Arabi L, Gholamzade Dewin A, Jafari M. ”5TR1 aptamer-PEGylated liposomal doxorubicin enhances cellular uptake and suppresses tumour growth by targeting MUC1 on the surface of cancer cells.” Artif Cells Nanomed Biotechnol. 2017 Dec

10. Razazan A, Behravan J, Arab A, Barati N, Arabi L, Gholizadeh Z, Hatamipour M, Reza Nikpoor A, Momtazi-Borojeni AA, Mosaffa F, Ghahremani MH, Jaafari MR.” Conjugated nanoliposome with the HER2/neu-derived peptide GP2 as an effective vaccine against breast cancer in mice xenograft model. PLoS One. 2017 Oct11.

11.Immunoengineering in glioblastoma imaging and therapy Zanganeh S, Georgala P, Corbo C, Arabi L, Ho JQ, Javdani N, Sepand MR, Cruickshank K, Campesato LF, Weng CH, Hemayat S, Andreou C, Alvim R, Hutter G, Rafat M, Mahmoudi M. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2019 Nov;

HONORS AND AWARDS:

Communication chair of Immuno Delivery Focus group at Controlled release Society since 2019

1. Rank 1st in poster presentation in translational Nanomedicine, The European and Global Summit for Clinical Nanomedicine and targeted medicine, 2-5 September 2018 (11th CLINAM 2018)
Basel- Switzerland

2. Highlights of Student Abstracts, 43rd Annual Meeting & Exposition of the Controlled Release Society (CRS), 2016, Seattle,
Washington, USA

3. Rank 1st in poster presentation, 14th Iranian Pharmaceutical Congress (IPSC2015) Dec 2015-
Tehran-Iran

4. Top Ph.D. student award in Research Festival of School of Pharmacy (Rank 2nd and 3rd), 2014 and 2015.

5. Awarded Ph.D. student of the School of Pharmacy of the Mashhad University of Medical Sciences for the highest quality original publication of the year (2014-2015)

6. Fellowship of CLINAM foundation for The European Summit for Clinical Nanomedicine 2015 (8th CLINAM 2015)
Basel- Switzerland

7. Fellowship of CLINAM foundation for The European Summit for Clinical Nanomedicine 2014 (7th CLINAM 2014)
Basel- Switzerland

8. Awarded Ph.D. student of the School of Pharmacy of the Mashhad University of Medical Sciences for the highest quality journal club presentation of the year (2013)

9. Fellowship of CLINAM foundation for The European Summit for Clinical Nanomedicine 2013 (6th CLINAM 2013)
Basel- Switzerland

10. Rank 1st for poster presentation in Biovalley Life Science Week- 25th September, 2012,
Basel- Switzerland

11. Rank 1st among candidates, Iranian National “pharmaceutical Nanotechnology” Ph.D. comprehensive Exam based on examination and interview 2011

12. Rank 1st among candidates, Iranian National “pharmaceutical Nanotechnology” Ph.D. Entrance Exam based on entrance examination and interview 2008

13. Rank 1st among Ph.D. student of the School of Pharmacy of the Mashhad University of Medical Sciences based on overall scores(18.9 out of 20)

14. Rank 2nd in Pharm.D. based on overall scores (18.05 out of 20)

15. Top 1% Nationwide (Rank 4th in the country) in the comprehensive basic sciences exam of pharmacy students, 2006.

16. Top 0.1% Nationwide entrance exam of Iranian Universities

PRESENTATIONS AT SCIENTIFIC MEETINGS:

ORAL PRESENTATIONS:

Arabi L, Jaafari M.R, "Enhancement of Tumor Penetration and Efficacy of Liposomal Doxorubicin: What We Have learned”,
International Conference on Nanomedicine and Nanobiotechnology – ICONAN, October 2019,
Munich, Germany

1. Arabi L, Badiee A, Mosaffa F, Jaafari M.R, "Targeting EpCAM marker with monoclonal antibody improves cellular uptake and antitumor activity of liposomal doxorubicin”, Oral presentation, ILS/LRD Liposome Advances Conference,16-18 September 2017,
Athens, Greece

2. Arabi L, Baschiera B, Wyttenbach S, Kustermann S, Ruiz C, Zippelius A, Roth A, Bubendorf L, “The use of patient derived malignant effusions as specific models for in-vitro assays for personalized cancer medicine”, Oral Presentation, 1st International Nastaran Cancer Symposium, October 1, 2015, Mashhad, Iran

3. Arabi L,Quagliata L, Rufle A, Terracciano L, Ruiz C and Baumhoer D. “Prognostic and therapeutic implications of the oncogenic miR-17-92 cluster in osteosarcoma”, Oral Presentation, The 1st Iranian Molecular Medicine Conference, March 2014, Mashhad University of Medical Sciences, Mashhad, Iran

4. Arabi L, Jaafari M.R.“ in vitro and in vivo Targeted Drug Delivery of Cancer Stem Cells Isolated from C26 Murine Colon Carcinoma Cell Line, by Doxorubicin Nanoliposomes Conjugated with CD44, CD133, EpCAM Monoclonal Antibodies” Oral Presentation, Dec 2012, Onco-Day, Department of Oncology, University Hospital Basel, Basel, Switzerland

5. Arabi L. Jaafari M.R. “Targeting Cancer Stem Cells with Nanoparticle-Enabled Therapies” Oral Presentation, Nov 2012, Research Festival, Institute for Molecular Pathology, University Hospital
Basel, Switzerland

6. Arabi L. Jaafari M.R. “in vitro and in vivo Targeted Drug Delivery of Cancer Stem Cells by Doxorubicin Nanoliposomes Conjugated with Monoclonal Antibodies” Oral Presentation, Sep 2012, Institute for Molecular Pathology, University Hospital Basel,
Basel, Switzerland

7. Arabi L, Malaekeh-Nikuei. B, Kazemi Oskuee. R, Dehshahri. A, Ramezani. M. “PolycationicNanoliposomes-Mediated Gene Transfer System Constructed from Polyethylenimines Modified with Bromoalkane and Acrylacte Derivatives” - Oral Presentation in English -5th International Controlled Release Conference of Iran (ICRC 2011)- October 2011-
Mashhad- Iran

8. Arabi L, Malaekeh-Nikuei. B, Kazemi Oskuee. R, Dehshahri. A, Ramezani. M- “Nanoliposmes Modified with Lipopolymers Constructed from Polyethyleneimine (10 kDa) and Bromoalkane Derivatives Used for Gene Delivery; Preparation and Characterization and Transfection In Vitro”,Oral Presentation12th Iranian Pharmaceutical Sciences Congress (IPSC), Aug 2010,
Zanjan, Iran.

POSTER PRESENTATIONS:

Arabi L, Janani,M. Badiee A, Jaafari M.R Improving Therapeutic Efficacy of PEGylated Liposomal Doxorubicin, The 2019 Controlled Release Society Annual Meeting & Exposition. September 2019.
Valencia-Spain

1. Arabi L, Janani,M. Badiee A, Jaafari M.R, "Targeting The Mucin-1 Marker In Colon Carcinoma Improves The Therapeutic Efficacy Of Liposomal Doxorubicin ", The European and Global Summit for Clinical Nanomedicine and targeted medicine, 2-5 Sep 2018 (11th CLINAM 2018) Basel- Switzerland

2. Arabi L, Badiee A, Mosaffa F, Jaafari M.R, "Targeting CD133 Marker Improves Cellular Uptake And Antitumor Efficacy Of Liposomal Doxorubicin", NanoDDS’16 14th Annual Nanomedicine and Drug Delivery Symposium, Sept 2016,
Johns Hopkins University, Baltimore, Maryland, USA

3. Arabi L, Badiee A, Mosaffa F, Jaafari M.R. “Significant improvement of cellular uptake and antitumor activity of liposomal doxorubicin by targeting with anti-CD44 monoclonal antibody in mice bearing C-26 colon carcinoma”, Iranian Pharmaceutical Congress (IPSC2015)-December 2015-Tehran-Iran

4. Arabi L, Badiee A, Jaafari M.R., “Significant improvement of pharmacokinetic and therapeutic efficacy of liposomal doxorubicin by targeting the CD44 marker in colon carcinoma”, The European Summit for Clinical Nanomedicine (8th CLINAM 2015) , June 28-July 1,2015, Basel- Switzerland

5. Arabi L, Badiee A, Jaafari M.R., “Anti-CD44 antibody conjugated liposomal doxorubicin for targeting the cancer stem-like cells in mice bearing C26 colon carcinoma”- ANFC2015-March2015-Kish island-Iran

6. Arabi L, Badiee A, Mosaffa F, JaafariM.R. “Targeting the Cancer Stem Cells and Cancer Stem-like Cells Isolated from Cell Line by Liposomal Nanoparticles Conjugated with Monoclonal Antibodies” The European Summit for Clinical Nanomedicine (7th CLINAM 2014) , June 23-26,2014, Basel- Switzerland

7. Ruiz C, Vlajnic T, Arabi L, Wyttenbach S, Baschiera B, Rothschild S, Kustermann S, Zippelius A, Roth A, Bubendorf L, “Abstract Cancer Research: The use of patient derived malignant effusions as in vitro models for a personalized healthcare-approach in anticancer therapy”, AACR Annual Meeting, April 2014,San Diego, CA, USA

8. Arabi L, Badiee A, Jaafari M.R. “Isolation and Identification of Putative Cancer Stem Cells in C26 Murine Colon Carcinoma Cell Line”, The 5th Ph.D student Conference, March 2014, MUMS,
Mashhad, Iran

9. Arabi L, Badiee A, Mosaffa F, Jaafari M.R. “Isolation and Identification of Putative Cancer

Stem Cells in C26 Murine Colon Carcinoma Cell Line”, The 1st Middle East Controlled Release Conference (MECRC 2014) and The 6th Iranian Controlled Release Society Conference (ICRC 2014), 25-27th February 2014, Tehran University of Medical Sciences,
Tehran, Iran

10. Arabi L, Malaekeh-Nikuei. B, KazemiOskuee. R, Dehshahri. A, Ramezani. M. “Polycationic Nanoliposomes as DNA Nanocarriers with Low Toxicity: A Promising Gene Transfer System for Further in vivo Study”, The European Summit for Clinical Nanomedicine (6th CLINAM 2013) , June 23-26,2013, Basel- Switzerland

11. Arabi L, Baschiera B, Wyttenbach S, Kustermann S, Ruiz C, Zippelius A, Roth A, Bubendorf L.“The use of patient derived malignant effusions as specific models for in-vitro assays”, 5th Freiburg Symposium on Anticancer Drug Discovery, April 24 – 27, 2013, Freiburg, Germany

12. Arabi L, Quagliata L, Rufle A, Terracciano L, Ruiz C and Baumhoer D. “Prognostic and therapeutic implications of the oncogenic miR-17-92 cluster in osteosarcoma”, Non-coding RNA, epigenetics and transgenerational inheritance, April 2013,
Churchill College in Cambridge University, Cambridge, UK

13. Quagliata L, Matter M, Arabi L, Piscuglio S, Makowska Z, Kovac M, Tornillo L, Heim M.H., Cillo C, Terracciano L, “HOTTIP Expression Levels Predict Patients’ Survival and Metastasis Formation In Hepatocellular Carcinoma” Charles RodolpheBrupbacher Symposium, January 30 - February 1, 2013, University Hospital Zurich, Zurich, Switzerland

14. Quagliata L, Matter M, Arabi L, Piscuglio S, Makowska Z, Kovac M, Tornillo L, Heim M.H., Cillo C, Terracciano L, “HOTTIP Expression Levels Predict Patients’ Survival and Metastasis Formation In Hepatocellular Carcinoma” Noncoding RNAs in Development and Cancer, January 20 - January 25, 2013, Vancouver, British Columbia, Canada

15. Arabi L, Stem Cells and Cancer. 7th International Heinrich F.C. Behr Symposium- October 14 - 16, 2012, German Cancer Research Center (Dkfz),
Heidelberg, Germany

16. Arabi L, MalaekehNikuei. B, KazemiOskuee. R, Dehshahri. A, Ramezani. M- “Preparation, characterization and transfection efficiency of polycationicnanoliposomes as DNA nanocarriers with low toxicity“Biovalley Life Science Week- 25th September- 2012-
Basel- Switzerland

17. Arabi L, Badiee.A, Mosaffa, F, Jaafari,M.R. “Cancer Stem Cells: Novel Targets of Anti-Cancer Treatments” -Poster Presentation-5th International Controlled Release Conference of Iran (ICRC 2011) - October 2011-
Mashhad- Iran

18. Arabi L, Iranian Congress On Biology and Applications of Stem Cells, 27-29 April, 2011, Institute Of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

19. Arabi L, Jaafari M.R. “Targeting Cancer Stem Cells and Their Microenvironmental Niche”- Poster Presentation- Research Festival- Avicenna Research Center, central lab, Mashhad University of Medical Sciences- December, 2011,
Mashhad, Iran

20. Arabi L, Jaafari M.R. “Novel Cancer Therapies by Targeting Cancer Stem Cells and Their Microenvironmental Niche”, Poster Presentation, Symposium On Molecular Aspects of Cancer, Instituto of Biotechnology, Ferdowsi University of Mashhad, December, 2010,
Mashhad, Iran

21. Arabi L, Malaekeh-Nikuei. B, KazemiOskuee. R, Dehshahri. A, Ramezani. M. “Preparation and Characterization of Nanoliposmes Modified with Lipopolymers Constructed from Polyethyleneimine and Bromoalkane Derivatives”, Poster Presentation, 6th Nanotechnology Iranian Student Conference, 16-18 December 2009, Tehran, Iran

22. Arabi L, Malaekeh-Nikuei. B, KazemiOskuee. R, Dehshahri. A, Ramezani. M. “Preparation and Characterization of Nanoliposmes Modified with Lipopolymers Constructed from Polyethyleneimine and Bromoalkane Derivatives”, Congress on Nanomedicines, Nanobiotechnology, Nanobiosensors and Nanomaterials in Medicine Sciences, Jul 2009, Mashhad, Iran

PROFESSIONAL DEVELOPMENT:

Assistant Editor of Iranian Journal of Medical Sciences (IF= 1.85)

MANUSCRIPT REVIEWS:

-Journal of Controlled Release

-Iranian Journal of Basic Medical Sciences

-Iranian Journal of Pharmaceutical Sciences

-Immunologic Research

-Experimental and Molecular Pathology

..

MEMBERSHIP IN PROFESSIONAL ASSOCIATIONS AND ACTIVITIES:

-Communication chair of Immuno Delivery Focus group at Controlled release Society

-Ambassador of Young scientist committee in Controlled release Society

-member of Controlled release Society

-member of the European Society for Nanomedicine

-Referee in 19th Iranian Pharmacy Students Seminar (IPSS19)- October 2015- Shiraz- Iran

-Member of scientific committee and referee in 5th International controlled release conference of Iran (ICRC 2011)- October 2011- Mashhad- Iran

-Member of scientific committee and referee in 8th nanotechnology Iranian student conference, December 2011, Mashhad, Iran

-Member of scientific committee and referee in 5th International controlled release conference of Medical Students of East of Iran

-Member of Student Research Committee, Mashhad University of Medical Science, pharmacy school, Since 2005, Mashhad, Iran

-Member of Talented and Gifted students center, Mashhad University of Medical Science, pharmacy school, Since 2002, Mashhad, Iran

WORKSHOPS:

1. Workshop on mechanisms of drug interactions based on clinical findings, by Dr. Akhlaghi (University of Rhode Island, USA)- 5th November 2015, Mashhad-Iran

2. Virtual workshop for training Referee, 19th Iranian Pharmacy Students Seminar (IPSS19)-March 2015

3. Patent Registration, BuAli research Center, 4th August 2015- Mashhad-Iran

-Pathway to publication workshop- Mashhad University of Medical Sciences-Nov 2014- Mashhad-Iran

4. Nanocarrier workshop by Prof. Theresa Allen (University of Alberta) -ANFC2015-Kish island-Iran

5. Tissue engineering, Tehran University of Medical Sciences, Feb 2014, Iran

6. Business Planning Workshop- Tarbiyat Modarres university-26 February 2014- Tehran-Iran

7. Cytation 3 Cell Imaging Multi-Mode Reader workshop- BuAli Research center- December 2014-Iran

8. Stem Cell Research in Academia and Industry, 23st BD-STCS Workshop, Oct 2012, Basel, Switzerland.

9. PCR-RFLP (restriction fragment length polymorphism), May 2012, BuAli Research Center, Medical genetics group, Medical School, Mashhad, Iran

10. Flow Cytometry and Cell Isolation Methods (MACS and FACS), 2011, Rouyan Inistitute, Tehran, Iran.

11. Patent Registration, 5th International Controlled Release Conference of Iran (ICRC 2011) –Oct 2011- Mashhad- Iran.

12. Principle of Flow Cytometry and Its Applications, Avicenna Research Center, Central Lab, Mashhad University of Medical Sciences, 13 July 2011, Mashhad, Iran.

13. Application of Nanotechnology in Medicine and Engineering, Professor Mansouri, Ferdowsi University Of Mashhad., Mashhad, Iran.

14. Treatment of Isolated Mesenchymal Stem Cells from Bone Marrow, Cord Blood and Adipose Tissue or Cell Therapy, 25-26 April 2011, Iranian Congress On Biology and Applications of Stem Cells, Ferdowsi University of Mashhad, Mashhad, Iran.

15. Preparation and Characterization of Nanogel, 12th Iranian Pharmaceutical Sciences Congress (IPSC 2010), 4 August 2010, Zanjan, Iran.

16. Scientific Articles Writing and Publishing Methods- 6th Nanotechnology Iranian Student Conference, 16-18 December 2009, Tehran, Iran.

17. Microsoft Office Programs (Outlook, April 2011- Excel, August 2010 -Onenote, September 2010- Powerpoint, 2010) Mashhad University of Medical Science, Pharmacy School, Mashhad, Iran.

18. Reference Management (Endnote & Reference Manager) 2009, Sept 2009, Mashhad University of Medical Science, Pharmacy School, Mashhad, Iran.

19. SPSS Data Analysis, September 2009, Mashhad University of Medical Science, Pharmacy School, Iran.

20. Electronic Resources and Databases, 2008, Student Research Committee, MUMS, Mashhad, Iran.

21. Herbal Medicine, 2008, Student Research Committee, MUMS, School of Medicine, Mashhad, Iran.

22. Traditional Medicine, 2008, Student Research Committee, MUMS, Mashhad, Iran.

23. Principles of Astronomy, January 2007, Ferdowsi University of Mashhad, Faculty of Science, Iran.

24. Introduction to Nanobiotechnology, Jul 2007, Student Research Committee, MUMS, Mashhad, Iran.

25. Introduction to Research and Research Methodology, 2006, Student Research Committee, MUMS, Iran

LANGUAGE: Native speaker in Persian, Fluent in English, Intermediate Proficiency in Arabic, A1/2 in German

REFERENCE:

Prof. Mahmoud Reza Jaafari, Nanotechnology department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran, [email protected]

Prof. Luigi Terracciano, Molecular Pathology Division, Institute of Pathology, University Hospital Basel, Switzerland, [email protected]

Prof. Theresa M. Allen, Professor Emeritus, Dept. of Pharmacology,

University of Alberta Adjunct Professor, Dept. of Biochemistry &Molecular Biology, University of British Columbia, [email protected]