Zeeba Kamaliyan

Zeeba Kamaliyan

Education:

B.S in Cell and Molecular Biology-Genetics, Shahid Bahonar University of Kerman, Kerman, Iran. 2009-2013.

M.Sc in Human Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2013-2016.

Ph.D in Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2017-2022.

Research Interests:

Cancer biology, Human genetics, Molecular genetics, Non-coding RNAs, Bioinformatics.

Wet Lab Skills:

DNA/RNA extraction from blood and tissue samples, qRT-PCR, Tetra-ARMS PCR, RFLP, Cell culture, MTT assay, Western Blot, Flow Cytometry.

Dry Lab Skills:

Primer design for coding and non-coding RNAs, Statistical analysis of expression/association data, WES interpretation, Microarray data analysis, Gene sequence analysis, and Bioinformatics analysis.

Proficient user of MS Office, Excel, SPSS, Graphpad Prism, Cytoscape, and experienced with genetic databases and in-silico tools.

Research Experiences:

Conducted bioinformatics approach for evaluating microarray data and gene expression profiling for Triple-negative Breast cancer.

Performed mRNA-miRNA-circRNA regulatory networks extraction by integrative analysis of multiple microarray datasets for Breast cancer.

Evaluated the expression level of selected mRNAs, lncRNAs, miRNAs and circRNAs by performing RT-qPCR followed by quantitative and qualitative data analysis in Breast, Colon, Gastric and Leiomyoma tumors.

Performed the association studies of multiple polymorphisms (case-control studies), from Tetra-ARMS PCR primer design to statistical analysis of the results in Colon cancer and Azoospermia.

Carried out a mutation screening assay for a piRNA pathway gene using M-SSCP electrophoresis followed by exon sequencing in Azoospermic patients.

Publications:

1. Zeeba Kamaliyan, Reza Mirfakhraie, Ghasem Azizi-Tabesh, Farzaneh Darbeheshti, Ramesh
 Omranipour, Nasrin Ahmadinejad, Elham Zokaei, Vahid-Reza Yassaee.
The role of FOXC1/FOXCUT/DANCR axis in triple negative breast cancer: a bioinformatics and experimental approach. Molecular Biology Reports. 2022. 49(4):2821–9. https://doi.org/10.1007/s11033-021-07093-3

2. GhasemAzizi-Tabesh, Zeeba Kamaliyan, Farzaneh Darbeheshti, Ramesh Omranipour, Vahid Soleimani, Nasrin Alipour, Reza Mirfakhraie, Vahid Reza Yassaee.
Overexpression of GABRP gene in triple-negative breast cancer: Molecular mechanisms and interpretation. International Journal of Cancer Management. 2021. 14(11): e119130. https://doi.org/10.5812/ijcm.119130

3. Farzaneh Darbeheshti, Elham Zokaei, Yaser Mansoori, Sima Emadi Allahyari, Zeeba Kamaliyan, Sepideh Kadkhoda, Javad Tavakkoly Bazzaz, Nima Rezaei, Abbas Shakoori.
Circular RNA hsa_circ_0044234 as distinct molecular signature of triple negative breast cancer: a potential regulator of GATA3. Cancer Cell International. 2021. 21(312). https://doi.org/10.1186/s12935-021-02015-6

4. Hossein Sadeghi, Zeeba Kamaliyan, Roohollah Mohseni, Unes Sahebi, Ehsan Nazemalhosseini-Mojarad, Naser Aghaei, Mohammad Reza Zali, Hamid Asadzadeh Aghdaei, Reza Mirfakhraie, Arfa Moshiri.
Dysregulation of vitamin D synthesis pathway genes in colorectal cancer: A case-control study. Journal of Clinical Laboratory Analysis. 2020. 35:e23617. https://doi.org/10.1002/jcla.23617

5. Zeeba Kamaliyan, Sara Pouriamanesh, Mohsen Soosanabadi, Milad Gholami, Reza Mirfakhraie.
Investigation of piwi-interacting RNA pathway genes role in idiopathic non-obstructive azoospermia.
Scientific Reports. 2018. 8(142). https://doi.org/10.1038/s41598-017-17518-4

6. Atieh Abedin-Do, Sara Pouriamanesh, Zeeba Kamaliyan, Reza Mirfakhraie.
Angiotensin converting enzyme gene rs4343 polymorphism increases susceptibility to migraine. CNS Neuroscience &Therapeutics. 2017. 23(8): 698-699. https://doi.org/10.1111/cns.12712

7. Samaneh Sadeghi, Mandana Khorrami, Mona Amin-Beidokhti, Maryam Abbasi, Zeeba Kamalian, Shiva Irani, Mirdavood Omrani, Ozra Azmoodeh, Reza Mirfakhraie.
The study of MED12 gene mutations in uterine leiomyomas from Iranian patients. Tumor Biology. 2016. 37(2):1567-71. https://doi.org/ 10.1007/s13277-015-3943-8