Mahbube Jafari

Education:

2015 – 2018   M.Sc. in Human Genetics

• Shahrekord University of Medical Sciences, Shahrekord, Iran
• Thesis Topic: “Sh-RNA Mediated NAGA Gene Silencing and Evaluation of the Effects on TNFR2 and
  a₂b₁ 
  Integrin Signaling Pathways in Cancer Cell Lines”
• Supervisor: Prof. Morteza Hashemzadeh Chaleshtori, Dr. Seyed Abbas Mirzaei
  
• GPA:  18.41/20

2010 – 2014   B.Sc. in Genetics

• University of Shahrekord, Shahrekord, Iran
• GPA:  17.59/20

Work Experience

· Position: Research assistant      July 2019-present

· Location: A. Immunodeficiency Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran

· Project Title: Preparation of Genetic Database for PID Patients

Publications

• Synergistic Effects of Lauryl Gallate and Tamoxifen on Human Breast Cancer Cells, IJPM (forthcoming)
• shRNA-mediated Downregulation of α-N-Acetylgalactosaminidase Can Affect Cancer-Related Cellular Signaling Pathways (in progress)

Laboratory Skills

• Lab Safety Principles (GLP)
• Polymerase Chain Reaction (PCR)
• Gel Electrophoresis
• Real-time Polymerase Chain Reaction
• Cloning
• Cell Culture
• Transfection
• Fluorescent & Light Microscopy
• Western Blotting
• Flow cytometry

• Karyotyping

• Bioinformatics

Bioinformatic Skills

• Bioinformatics and genomics databases (NCBI, Ensembl, UniProt, and EMBL-EBI database)
  
• Primer designing

• siRNA/shRNA designing

• Applications: Microsoft office package, Gene Runner, Rest, Vector NTI, ImageJ, GraphPad Prism, SPSS, Endnote, Mendeley

Research Interests

• Human Genetics
• Cancer Genetics
• Immunogenetics
• Bioinformatics and Genomics (Next Generation Sequencing Technologies)
• Gene Therapy & Gene Editing
• Cytogenetics
• Genetic and Epigenetic Related Disease
• Gene Expression Analysis at Transcription Level (Transcriptomics)
  
• Gene Expression Analysis at Translation Level (Proteomics)
• Non-Coding RNAs & miRNAs

Honors

• B.Sc. Ranked 3^rd among 40 bachelor students as an exceptionally talented student, Shahrekord University, Shahrekord, Iran
• M.Sc. Talented student, Shahrekord University of Medical Sciences, Shahrekord, Iran
• Ranked 1^st in seminar presentation among M.Sc. and Ph.D. students, with the title of “The Role of α-NaGalase Enzyme in Cancer Progression”, 2016,
  Cellular and molecular research center, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Seminar & Congresses

• 2^nd International and 14^th Iranian Genetics Congress, Tehran, Iran. May 21-23 2016

• Participation in “Non-coding RNAs and Diseases” Symposium, Shahrekord, Iran, November 26, 2017

• Poster presentation as first author entitled "Synergistic Effects of Lauryl Gallate and Tamoxifen on Human breast Cancer Cell", 3^rd International and 15^th Iranian Genetics Congress, Tehran, Iran. May 13-15 2018
  

• Poster presentation as the first author entitled “The Role of α–N-acetylgalactosaminidase enzyme in EPG 85/257 RDB Cell Line Signaling Pathways”,
  6^th International and 15^th Iranian International Congress of Biochemistry and Molecular Biology, Isfahan, Iran. August 25-28 2018

• Two days of PCR-SSCP method workshop, 2015, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
• Five days of Molecular diagnostic methods workshop, 2015, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
  
• The cytogenetic three-day workshop, 2015, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
  
• Three days of Cloning workshop, 2016, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran.
  
• Two-day Western Blot workshop, 2017, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran
• Two days of computational biology workshop, 2017, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran
• Two-day SPSS workshop, 2018, Cellular and molecular research center, Shahrekord University of medical science, Shahrekord, Iran