Shima Shekarchi

EDUCATION:
Master of Science in Anatomical Sciences (Gross Anatomy, Micro Anatomy, Histology and Embryology), Guilan
University of Medical Sciences, Iran | Sep 2016– Nov 2020 |
Thesis Title : Dimethyl fumarate prevents cytotoxicity and apoptosis mediated by oxidative stress in human
adipose‑derived mesenchymal stem cells

SUMMARY ABOUT ME:
Recent MSc graduate in Anatomical Sciences with 5 years of
research experience and several publications on Cell Therapy,
Regenerative Medicine, Cancer Biology, and Developmental Biology,
as well as 5 years of laboratory technician, laboratory manager and 1
year of professional experience as a research assistant, teaching
assistant, and class coordinator. Expert in laboratory techniques like stem cell technology, cell culture, and molecular techniques, along with software skills inclusive of presentation software and databases. Eagerly passionate about learning and excelling in Medical Research.

1- Cell Culture
Primary Cell Culture, Mammalian Cell Culture, Insect Cell Culture, Three-Dimensional (3D) Cell Culture, Bacterial
Cell Culture, CRISPR-Cas9, Oocyte, Sperm and Embryo Culture
2- Molecular/ Biochemical/ Immunological Techniques
Cytotoxicity and Apoptosis Detection Assays, Sample Preparation & RNA Extraction, Real-time PCR, ELISA,
Immunocytochemistry, Protein Purification, SDA-PAGE Electrophoresis, Western Blot
3- Stem Cell Technology
Isolation, Characterization and Expansion of Hematopoietic and Mesenchymal Stem Cells from a variety of
sources. Stem Cells Differentiation Methods and Staining

4- Medical Laboratory Technologist at Al-Zahra Hospital, Rasht, Iran , July2016- Dec 2021
I am expert in numerous areas including Chemistry, Hematology, Bacteriology, Serology, Fluid Analysis, Blood Bank
and Histology
4- Night Shift Manager at Medical Laboratory of Al-Zahra Hospital, Rasht, Iran , July2018- Dec 2021
5- Member of Accreditation Committee at Guilan University of Medical Sciences, Rasht, Iran,
from Dec 2021 Up to Now

PUBLICATION:
1- Shima Shekarchi , Amaneh Mohammadi Roushandeh , Mehryar Habibi Roudkenar , Mohammad Hadi
Bahadori, Dimethyl fumarate prevents cytotoxicity and apoptosis mediated by oxidative stress in human
adipose‑derived mesenchymal stem cells. Mol Biol Rep,2021 Sep;48(9):6375-6385
DOI: 10.1007/s11033-021-06638-w
2- Sahar Evazi Bakhshi , Amaneh Mohammadi Roushandeh , Mehryar Habibi Roudkenar , Shima Shekarchi,
Mohammad Hadi Bahadori, CRISPR/Cas9-mediated knockout of HO-1 decreased the proliferation and migration of
T47D cells and increased cisplatin-induced apoptosis: an in vitro study , Med Oncol,2022 Aug 16;39(11):175.
DOI: 10.1007/s12032-022-01773-1
3- Nima Najaf‑Ghalehlou, Alireza Feizkhah , Mohammadreza Mobayen ,Zahra Pourmohammadi‑Bejarpasi ,
Shima Shekarchi , Amaneh Mohammadi Roushandeh, Mehryar Habibi Roudkenar. Plumping up a Cushion of
Human Biowaste in Regenerative Medicine: Novel Insights into a State‑of‑the‑Art Reserve Arsenal. Stem Cell
Reviews and Reports, volume 18, pages2709–2739 (2022). DOI: 10.1007/s12015-022-10383-3
4- Chia Bamshad; Amaneh Mohammadi Roushandeh; Zahra Pourmohammadi-Bejarpasi; Fatemeh Amiri;
Shima Shekarchi; Ali Jahanian Najafabadi,Platelets Emerging As a State-Of-The-Art Arsenal for Mitochondria
Transplantation in Rodent Models of Traumatic Brain Injury. Brain Injury (Under review)
MEMBERSHIP
1- Executive Member
First Symposium of Stem Cell and Regenerative Medicine, 5-6 Dec 2017,Guilan University of Medical Sciences,
Rasht, Iran
2- Executive Member
Dr. Hakim Zadeh's Second National Habitat Conference with Focus on Rehab and Trauma, 25 Oct 2018, Guilan
University of Medical Sciences, Rasht, Iran
3- Executive Member
Workshop Tissue Engineering & Regenerative Medicine, 14-15 April 201, Guilan University of Medical Sciences,
Rasht, Iran
WORKSHOP AND SEMINARS
1- First Symposium of Stem Cell and Regenerative Medicine, 5-6 Dec 2017,Guilan University of Medical
Sciences, Rasht, Iran, Participant
2- Dr. Hakim Zadeh's Second National Habitat Conference with Focus on Rehab and Trauma, 25 Oct 2018,
Guilan University of Medical Sciences, Rasht, Iran, Participant
3- Workshop Tissue Engineering & Regenerative Medicine, 14-15 April 201,Guilan University of Medical
Sciences, Rasht, Iran, Participant
4- Transcranial Direct Current Stimulation (tDCS) Workshop was held in Dr. Hakim Zadeh's Second
National Habitat Conference with Focus on Rehab and Trauma, 25 Oct 2018, Guilan University of Medical
Sciences, Rasht, Iran, Participant
5- Webinar of The Basis of Anesthesia and Analgesia in Laboratory Animals, 19 Feb 2022, Guilan University
of Medical Sciences, Rasht, Iran, Participant

REFRENCES
1- Mehryar Habibi Roudkenar, Professor in Medical Biotechnology, Department of Medical Biotechnology,
Guilan University of Medical Sciences AND Burn and Regenerative Medicine Research Center, Velayat
Hospital, Guilan University of Medical Sciences, Rasht, Iran
Email: [email protected] [email protected]
2-Amaneh Mohammadiroushandeh , Phd in Anatomical Sciences, School of Biomedical Sciences, Faculty of Medicine and Health, University of New South Wales(UNSW), Sydney, Australia
Email: [email protected] [email protected]