Farshid Zandsalimi

Education

Ph.D. candidate in Molecular Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran (GPA: 4/4).
2019-Now

Thesis: Evaluating the effect of truncated forms of RUNX3 on the viability and apoptosis of MCF-7 and AGS cell lines.

Supervisor: Dr. Mohammad Hossein Ghahremani

MSc in Microbial Biotechnology, University of Tehran (UT), Tehran, Iran. (GPA: 3.5/4).2016-2018

Thesis: Secretory expression of human Activin A growth factor through the
Twin-arginine translocation (Tat) pathway.

Supervisor: Dr Zahra Hajihassan



Bachelor of Science in Biotechnology, University of Kurdistan (UOK), Sanandaj, Iran. (GPA:3/4)




Publications


1- Ashrafizadeh M, Aghamiri S, Tan SC, Zarrabi A, Sharifi E, Rabiee N, et al. Nanotechnological Approaches in Prostate Cancer Therapy: Integration of engineering and biology.
Nano Today. 2022; 45:101532.

2- Abdolhosseini, M, Zandsalimi, F, Salasar Moghaddam, F, Tavoosidana, Gh. A review on colorimetric assays for DNA virus detection.,
Journal of Virological Methods. 301, 114461 (2022).

3- Aghamiri S, Zandsalimi F, Raee P, Abdollahifar M-A, Tan SC, Low TY, et al. Antimicrobial peptides as potential therapeutics for breast cancer.
Pharmacol Res. 2021 Sep 1; 171:105777.

4- Zandsalimi F, Talaei S, Ahari MN, Aghamiri S, Raee P, Roshanzamiri S, et al. Antimicrobial peptides: a promising strategy for lung cancer drug discovery?
Expert Opin Drug Discov.
2020 Nov 1;15(11):1343–54.

5- Zandsalimi F, Aghamiri S, Roshanzamiri S, Shahmohamadnejad S, Ghanbarian H. The emerging role of cold atmospheric plasma in glioblastoma therapy.
Plasma Process Polym. 2020 Oct 1;17(10):1900189.

6- Aghamiri S, talaei S, Roshanzamiri S, Zandsalimi F, Fazeli E, Aliyu M, et al. Delivery of genome editing tools: A promising strategy for HPV-related cervical malignancy therapy.
Expert Opin Drug Deliv.
2020 Jun 2;17(6):753–66.

7- Aghamiri S, Talaei S, Ghavidel AA, Zandsalimi F, Masoumi S, Hafshejani NH, et al. Nanoparticles-mediated CRISPR/Cas9 delivery: Recent advances in cancer treatment.
J Drug Deliv Sci Technol. 2020 Apr 1; 56:101533.

8- Zandsalimi F, Hajihassan Z, Hamidi R. Denovo designing: a novel signal peptide for tat translocation pathway to transport activin A to the periplasmic space of E. coli.
Biotechnol Lett2019 421. 2019 Nov 2;42(1):45–55.

9- Hajihassan Z, Khairkhah N, Zandsalimi F. Enhanced periplasmic expression of human activin A in Escherichia coli using a modified signal peptide.
Prep Bioche. Biotechnol. 2019 Feb 7;50(2):141–7.

10- Aghamiri S, Jafarpour A, Zandsalimi F, Aghemiri M, Shoja M. Effect of resveratrol on the radiosensitivity of 5-FU in human breast cancer MCF-7 cells.
J Cell Biochem. 2019 Sep 1;120(9):15671–7.

Awards and Honors


● Received full scholarship from Tehran University of Medical Sciences for Ph.D program, 2019.

● Received full scholarship from University of Tehran for master program, 2015.

● Ranked 8th among more than 2000 participants in the Iranian University Entrance Exam for

master's program in Biotechnology, 2015.

● Ranked 11th among 150 participants in the 20th
biology Scientific Olympiad for University

Students, 2015.

● Received full scholarship from University of Kurdistan for bachelor program, 2011.

● Ranked within the top 0.7% among more than 1.000.000 participants in the Iranian University

Entrance Exam for bachelor program, 2011.



Academic Projects


RUNX proteins and the Epithelial-Mesenchymal Transition (EMT) process. 2020-Now

● Investigating the role of RUNX3 in metastasis of AGS cells.

● Assessing the association between RUNX3 and the antiproliferative effect of TGF-β on MCF-7 cells.

Investigation of the relationship between CCR5Δ32 and HLA-G polymorphisms with the complications of Hepatitis B virus infection. 2018-2019

● Genotyped the patients with Hepatitis B virus infection for detection of CCR5Δ32 and HLA-G polymorphisms.

● Compared the complications of Hepatitis B virus infection.

The periplasmic expression of human activin A in E. coli
using a Denovo designed signal peptide. 2017-2018

● Designed a new TAT-dependent signal peptide to the periplasmic expression of recombinant human Activin A in
E. coli.

● Improved the folding structure of recombinant human Activin A.



Work Experience

Fermentation specialist, Behdad Genetic Co, Tehran, Iran.
2020-2021

● Preparation of inoculum.

● Implemented clean-in-place (CIP) and sterilize-in-place (SIP) systems.

● Set up and operate industrial-scale production fermenter.



Research Experience

Students’ Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.2019-2020

● Studied the use of antimicrobial peptides as potential therapeutic agents for cancer therapy.

● Investigated the latest methods for delivery of genome editing tools.



Teaching Experience

Teaching assistant, Tehran University of Medical Sciences.
2022

Teaching assistant, University of Tehran, Tehran, Iran. 2016



Laboratory Skills

● Gene Cloning ● Gel Electrophoresis ● Recombinant Protein Expression ● Protein Purification ● SDS-PAGE ● Western Blot ● Sequencing

● DNA/RNA Extraction ● Cell Culture ● PCR-Based Amplification



Computer Skills

● Molecular Dynamic (MD) Simulation ● Molecular Docking ● FoldX ● YASARA ● GROMACS ● PyMOL ● Discovery Studio ● Oligo 7



Language Skill

● IELTS: 7 (Listening: 7.5, Reading: 7, Speaking: 7, Writing: 6).